Lambda 10 3 filter wheel

An Olympus IX71 confocal fluorescence microscope with a 60×/1.42 oil objective was used to analyze the fluorescent protein expressing strains of Synechococcus sp. PCC 7002. The culture samples (1.5 mL) were centrifuged at 5000×g for 5 min, and the cell pellets were resuspended in approximately 50 µL of supernatant to concentrate the culture. A 10 µL aliquot of each culture was placed on a glass microscope slide, covered with a no. 1.0 cover slip, and sealed with nail polish. A Prior Scientific Lumen 200PRO fluorescence illumination system with a Sutter Instrument Lambda 10-3 filter wheel was used to excite the samples. The Chroma Chl LP filter cube (Em > 600 nm) with 484 nm excitation was used to detect chlorophyll-a (Chl-a) fluorescence; the Semrock GFP-3035B-OMF-ZERO (Em 520/35 nm) filter cube with 484 or 500 nm excitation was used to detect hGFP and Ypet, respectively; and the Olympus DSU-MRFPHQ (Em 597.5/55 nm) filter cube with 534 nm excitation was used to detect mOrange. SlideBook 6 software was used for image acquisition. The images were imported into ImageJ [37 ], upon which Chl-a fluorescence was colored red; fluorescent protein fluorescence was colored green; and scale bars were added.