Potassium ferricynaide

Reagents and their sources were as follows: phosphate buffer saline (PBS) (PAA Laboratories, Cölbe, Germany), sodium carbonate, ammonium bicarbonate, thiourea, dithiothreitol (DTT), urea, trypsin, trifluoroacetic acid‎ (TFA) (Sigma-Aldrich), CHAPS (AppliChem, Darmstadt, Germany), Acetonitril (ACN) (Promochem, Wesel, Germany), Immobilized pH gradient strips (IPG strips), ampholytes, protein assay kit (Bio-Rad, Munich, Germany), protease and phosphatase inhibitor cocktails (Roche, Mannheim, Germany), sodium dodecyl sulfate (SDS) (Serva, Heidelberg, Germany), potassium ferricynaide, Glycerin, sodium thiosulfate (Merck, Darmstadt, Germany), formic acid (BASF, Ludwigshafen, Germany), bromophenol blue (Carl Roth, Karlsruhe, Germany).

Cell culture media Dulbecco's Modified Eagle's medium (DMEM), fetal calf serum (FCS), phosphate buffer saline (PBS), penicillin and streptomycin were purchased from PAA Laboratories, Pasching, Austria. Fluorescein isothiocyanate-conjugated dextran (FITC-dextran), 4 kDa (FD4), Trypsin, MPA, and DMSO were purchased from Sigma-Aldrich, Steiheim, Germany. Protease and phosphatase inhibitor cocktails were purchased from Roche, Mannheim, Germany. Bromophenol blue was obtained from Carl Roth, Karlsruhe, Germany. Sodium dodecyl sulfate (SDS) was obtained from Serva, Heidelberg, Germany. Glycerin, potassium ferricynaide, and sodium thiosulfate were purchased from Merck, Darmstadt, Germany. Formic acid was purchased from BASF, Ludwigshafen, Germany. Magnesium chloride (MgCl₂), M-MLV RT enzyme, and 5X PCR buffer were from Invitrogen, Karlsruhe, Germany. Deoxynucleotide triphosphates (dNTPs) were from Roche, Mannheim, Germany and PCR primers were synthesized by Eurofins, Ebersberg, Germany. Ribonuclease (RNAase) inhibitor was obtained from Promega, Mannheim, Germany. The cell lysis buffer (10X) was obtained from Cell Signaling Technology, Danvers, MA, USA. All other chemicals used in this work were from the highest available purity from commercial sources unless otherwise stated.