All immunohistochemistry (IHC) staining was performed at the Boyuan Biological Laboratory. Whole section slides of FFPE tumor and paratumor were stained using a Dako instrument with PD‐L1 (bs‐1103R, Bioss, 1:200 dilution), IDO (13268‐1‐AP, Proteintech, 1:200 dilution), CD8(bs‐10699R, Bioss, 1:200 dilution), TIM‐3 (bs‐10699R, Bioss, 1:200 dilution) and FOXP3+ (bs‐10699R, Bioss, 1:200 dilution) antibodies. Whole section slides of FFPE lymph node were stained on a Dako instrument with PD‐L1 (bs‐1103R, Bioss, 1:200 dilution), IDO (13268‐1‐AP, Proteintech, 1:200 dilution) antibodies. All indices including PD‐L1, IDO, TIM‐3, CD8+, and FOXP3+ were stained Individually with corresponding antibody. The final staining results of the target protein were seen as a brown color, and the nucleus was blue‐purple.
Zhang S., Cai H., Huang J, & Wang G. (2022). Impact of the combination of sintilimab and chemotherapy on the tumor and paratumor PD‐L1, IDO, TIM‐3, FOXP3+ and CD8 expressions in patients with advanced esophageal squamous cell carcinoma. Thoracic Cancer, 13(23), 3284-3294.
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