Primary antibodies for β-actin (#12620), phospho (Thr202/Tyr204) ERK (#4370), and ERK (#9102), were from Cell Signaling Technology (Danvers, MA, USA). Antibodies for glutamic acid decarboxylase 65 (GAD65; SC-377154) and Tbr1 (SC-376258; Western blot) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies for Neu-N (MAB377) and Tbr2 (AB15894) were from Millipore (Burlington, MA, USA). The antibody for Ki67 (550609) was obtained from BD Pharmingen (San José, CA, USA). Antibodies for Sox2 (ab97959), Tbr1 (ab31941; immunofluorescence), vGlut1 (ab77822) and Pax6 (ab5790) were from Abcam Inc. (Cambridge, MA, USA). Secondary fluorescent antibodies were obtained from Jackson ImmunoResearch Co. Laboratories (West Grove, PA, USA). Polyvinylidene difluoride (PVDF), membranes and molecular weight standards for Western blot were obtained from BIO-RAD (Hercules, CA, USA). The enhanced chemiluminescence (ECL) Western blotting system was from Thermo Fisher Scientific Inc. (Piscataway, NJ, USA). Zinquin, the antibody for γ amino butyric acid (GABA; A2052), and all other reagents were of the highest quality available and were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA).
Enhanced chemiluminescence ecl western blotting system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Enhanced chemiluminescence (ECL) Western blotting system is a laboratory equipment used for the detection and analysis of proteins in biological samples. It utilizes a chemiluminescent detection method to visualize the target proteins on a membrane.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Cell Signaling Technology (2 mentions)
Ab77822, by Abcam (1 mentions)
Ab5790, by Abcam (1 mentions)
Ab97959, by Abcam (1 mentions)
Ab15894, by Merck Group (1 mentions)
Zinquin, by Merck Group (1 mentions)
Ab129068, by Abcam (1 mentions)
Ab133303, by Abcam (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
Anti rabbit ab6721, by Abcam (1 mentions)
3 protocols using enhanced chemiluminescence ecl western blotting system
1
Antibody Characterization for Neurodevelopment
2
Analytical Methods for Metabolic Biomarkers
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Cholesterol and triglyceride concentrations were determined using kits purchased from Wiener Lab Group (Rosario, Argentina). Glucose levels were measured using a kit purchased from Sigma-Aldrich Co (St. Louis, MO). Concentrations of insulin, glucagon-like peptide-1 (GLP-1), gastric inhibitory polypeptide (GIP), leptin and adiponectin were determined using kits purchased from Crystal Chem Inc. (Downers Grove, IL). Antibodies for monocyte chemoattractant protein-1 (MCP-1) (#2029), TNFα (#11948), phospho (Ser176/180) - IKKα/β (#2697), IKKα (#2682), JNK2 (#9258) and β-actin (#12620) were obtained from Cell Signaling Technology (Danvers, MA). Antibodies for F4/80 (sc-25830), nitric oxide synthase 2 (NOS2) (sc-649), phospho (Thr183/Tyr185) - JNK (sc-6254) and NOX3 (sc-67005) were from Santa Cruz Biotechnology (Santa Cruz, CA). Antibodies for 4-hydroxynonenal (4-HNE) (ab46545), NOX4 (ab133303) and gp91phox (ab129068) were from Abcam, Inc. (Cambridge, MA). The antibody for PTP1B (ABS40) was from EMD Millipore (Hayward, CA). PVDF membranes were obtained from BIO-RAD (Hercules, CA). The Enhanced chemiluminescence (ECL) Western blotting system was from Thermo Fisher Scientific Inc. (Piscataway, NJ). All other chemicals were purchased from Sigma-Aldrich Co (St. Louis, MO). The AC-rich blend was provided by NSE Products, Inc. (Provo, UT) and its composition is shown in Supplemental Table 1 .
3
Antibody Sources for Western Blot
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Primary antibodies for β-actin (#12620), phosphor (Thr180/Tyr182) p38 (#4511), p38 (#9212), phosphor (Thr18/Ser19) MLC (#3671), MLC (#3672), and PAR-2(#6976) were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies for ZO-1 (ab216880), Occludin (ab167161), MLCK (EP1458Y), AQP3 (ab153694), secondary antibodies including anti-rabbit (ab6721), and anti-mouse (ab6728) IgG antibodies conjugated to horseradish peroxidase were obtained from Abcam Inc (Cambridge, MA, USA). Antibodies for 5-HT3R and AQP8 were purchased from Bioss Inc (Beijing, China). Enhanced chemiluminescence (ECL) Western blotting system was acquired from Thermo Fisher Scientific Inc (Piscataway, NJ, USA).
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