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Recombinant human tnfα

Manufactured by Miltenyi Biotec
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Sourced in Germany

Recombinant human TNFα is a cytokine produced in E. coli. It is a key mediator of inflammatory and immune responses.

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Lab products found in correlation

Nf κb pathway sampler kit, by New England Biolabs (2 mentions) Anti pser 43 nemo, by Abcepta (2 mentions) Anti ubiquitin, by Agilent Technologies (2 mentions) Anti gsk 3β, by New England Biolabs (2 mentions) Anti tubulin and nonimmune iggs, by Thermo Fisher Scientific (2 mentions) Appropriate secondary antibody, by Jackson ImmunoResearch (1 mentions) Percp cy5, by BioLegend (1 mentions) Human igg control antibody, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Lipopolysaccharide lps, by Merck Group (1 mentions) Bay11 7082, by Merck Group (1 mentions) Anti ubiquitin antibody, by Agilent Technologies (1 mentions) Ag1478, by Merck Group (1 mentions) Sp60025, by Merck Group (1 mentions) Akt1 2 kinase inhibitor, by Merck Group (1 mentions) G 6976, by Merck Group (1 mentions) Brefeldin a, by Merck Group (1 mentions) N acetylcysteine, by Merck Group (1 mentions) Ionomycin, by Merck Group (1 mentions) Ly294002, by Merck Group (1 mentions)

5 protocols using recombinant human tnfα

1

Macrophage Activation and Endothelial Dysfunction

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Recombinant human TNF-α and human macrophage colony-stimulating factor (M-CSF) was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Human oxidized low density lipoprotein (oxLDL), Dil(1,1´-dioctadecyl-3,3,3`,3`-tetramethylindocarbo-cyanine perchlorate)-labelled oxLDL and native LDL (nLDL) was from Kalen Biomedical LLC (Montgomery Village, MD). Antibodies for Western blot against ICAM-1, E-selectin were obtained from R&D Systems (Minneapolis, MN) and VCAM-1, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Santa Cruz (Dallas, TX). An antibody for immunofluorescence against VE-cadherin was obtained from Santa Cruz. CD11b and CD11c antibodies labelled with allophycocyanin (APC) and PerCP/Cy5.5 respectively for flow cytometry were obtained from BioLegend (San Diego, SA). Appropriate secondary antibodies were from Jackson ImmunoResearch (West Grove, PA) Adalimumab (HUMIRA) was obtained from Abbott GmbH (Wiesbaden, Germany). Human IgG control antibody, 4',6-diamidino-2-phenylindole (DAPI) and lipopolysaccharide (LPS) were obtained from Sigma-Aldrich (Munich, Germany).
Oberoi R., Schuett J., Schuett H., Koch A.K., Luchtefeld M., Grote K, & Schieffer B. (2016). Targeting Tumor Necrosis Factor-α with Adalimumab: Effects on Endothelial Activation and Monocyte Adhesion. PLoS ONE, 11(7), e0160145.
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2

Antibody Panel for NF-κB Signaling

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Antibodies against TRAF1, TRAF2, TRAF3, TRAF6, p50, p52, p105, p100, IκBα, the phosphorylated forms of IKKα (Ser176) β (Ser177), p65 (Ser536), c-jun (Ser63) and IL-6 were purchased from Cell Signaling Technology. Antibodies against p65 and TRAF5 were obtained from Santa Cruz Biotechnology. The antibody against EVER2 was purchased from Interchim. The anti-lamin B antibody was obtained from Thermo Scientific. Recombinant human TNFα was purchased from Miltenyi Biotec. Anti-actin, anti-Flag and anti-hemaglutinin (HA) antibodies, PMA, ionomycin, brefeldin A, Ly294002, SP60025, Bay 11-7082, Gö6976, AG1478, Akt1/2 kinase inhibitor and N-acetylcysteine were obtained from Sigma-Aldrich. Anti-Gluc and anti-Gfp antibodies were obtained from BioLabs. Anti-KL1 antibody was purchased from Immunotech Biotechnologies. Anti-ubiquitin antibody was obtained from Dako.
Vuillier F., Gaud G., Guillemot D., Commere P.H., Pons C, & Favre M. (2014). Loss of the HPV-Infection Resistance EVER2 Protein Impairs NF-κB Signaling Pathways in Keratinocytes. PLoS ONE, 9(2), e89479.
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3

Antibodies for NF-κB Pathway Analysis

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The following antibodies were used: NF-κB Pathway Sampler Kit (#9936) (New England BioLabs, Frankfurt/Main, Germany), anti-pSer-43-NEMO (Abgent, Heidelberg, Germany), phospho-specific anti-pSer-43-NEMO (Eurogentec, Köln, Germany), anti-Ku70 (#sc-1487), anti-DNA-PKcs (#sc-5282) (Santa Cruz Biotechnology, Heidelberg, Germany), anti-Ku70/Ku80 (Abcam, Cambridge, UK), anti-ubiquitin from DAKO (Hamburg, Germany), anti-IKKγ/NEMO (#I5032), anti-IKKγ/NEMO (#WH0008517M1), anti-β-actin (Sigma-Aldrich, Germany), and anti-UK (Invitrogen, Germany). Recombinant human TNFα was purchased from Miltenyi (Bergisch Gladbach, Germany), and the DNA-PK inhibitor NU7026 was purchased from Calbiochem (La Jolla, CA, USA).
Medunjanin S., Putzier M., Nöthen T., Weinert S., Kähne T., Luani B., Zuschratter W, & Braun-Dullaeus R.C. (2020). DNA-PK: gatekeeper for IKKγ/NEMO nucleocytoplasmic shuttling in genotoxic stress-induced NF-kappaB activation. Cellular and Molecular Life Sciences, 77(20), 4133-4142.
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4

Analysis of NF-κB Signaling Pathway

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The following antibodies and reagents were used: anti-GSK-3β (#9832) and an NF-κB Pathway Sampler Kit (#9936) (New England Biolabs, Frankfurt/Main, Germany), phosphor-specific anti-pSer-31-NEMO and anti-β-actin (Abcam, Cambridge, UK), anti-Vps4A (#SAB4200025) and mouse anti-FLAG M2 (Sigma-Aldrich, Taufkirchen, Germany), and anti-tubulin and nonimmune IgGs (Invitrogen, Darmstadt, Germany). Recombinant human TNFα was purchased from Miltenyi (Bergisch Gladbach, Germany), bafilomycin A (#B1793) was purchased from Sigma-Aldrich, and the GSK-3β inhibitor SB216730 was purchased from Calbiochem (San Diego, CA, USA).
Wackernagel L.M., Abdi Sarabi M., Weinert S., Zuschratter W., Richter K., Fischer K.D., Braun-Dullaeus R.C, & Medunjanin S. (2022). IKKγ/NEMO Localization into Multivesicular Bodies. International Journal of Molecular Sciences, 23(12), 6778.
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5

Signaling Pathway Antibody Panel

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The following antibodies were used: anti-GSK-3β (#9832), anti-K48-linked ubiquitin (#8081), NF-κB Pathway Sampler Kit (#9936), anti-TNF-R1 (#C25C1) and anti-RIP (#D94C12) (New England BioLabs, Frankfurt/Main, Germany), anti-Ubiquitin from DAKO (Hamburg, Germany), anti-pSer-31-NEMO, anti-TRAF6, anti-RIP1 and anti-β-actin (Abcam, Cambridge, UK); anti-p47 (Abnova, Heidelberg, Germany); phospho-specific anti-pSer-8-NEMO and anti-pSer-17-NEMO (Eurogentec, Köln, Germany); anti-FLAG M2 mouse (Sigma-Aldrich, Taufkirchen, Germany); anti-pSer-43-NEMO (Abgent, Heidelberg, Germany); anti-Tubulin and non-immune IgGs (Invitrogen, Darmstadt, Germany). Recombinant human TNFα was from Miltenyi (Bergisch Gladbach, Germany), GSK-3 inhibitors SB216730 and AR-A014418 from Calbiochem (La Jolla, CA, USA).
Medunjanin S., Schleithoff L., Fiegehenn C., Weinert S., Zuschratter W, & Braun-Dullaeus R.C. (2016). GSK-3β controls NF-kappaB activity via IKKγ/NEMO. Scientific Reports, 6, 38553.
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