Rat antimouse F4/80 (MCA497R, a marker of macrophages) and Ly-6G (Gr-1, MCA2387, a marker of neutrophils) were purchased from AbD Serotec (now Bio-Rad Laboratories, Hercules, CA); mouse anti–mannose receptor (CD206, MAB25341) and kidney injury molecule 1 (a marker of renal tubular injury, MAB1817) were from R&D Systems (Minneapolis, MN); affinity-purified rabbit antihuman IL-4Rα (NBP1-00884) was from Novus Biologicals (Littleton, CO); goat antiarginase 1 (ab60176) and rabbit anti-CX3CL1 (ab25088) were from Abcam (Cambridge, UK); goat antihuman connective tissue growth factor (SC-14939), IL-4 (SC-1260), IL-13 (SC-1292), pSTAT6, and rabbit antinitrotyrosine (SC-55256) were from Santa Cruz Biotechnology (Santa Cruz, CA); rabbit antimurine collagen type I (600-401-103-01) was from Rockland Immunochemicals (Limerick, PA); mouse anti-α-smooth muscle actin (A5228, a marker of myofibroblasts) was from Sigma (A5228); and pSTAT5 and rabbit anti–high mobility group box 1 (a marker of secondary necrosis) were from Cell Signaling Technologies (Danvers, MA).
Rat antimouse f4 80 mca497r
Manufactured by Bio-Rad
Sourced in United Kingdom
The Rat antimouse F4/80 (MCA497R) is a lab equipment product from Bio-Rad. It is an antibody used for the detection and identification of the F4/80 antigen, which is expressed on the surface of mouse macrophages.
Automatically generated - may contain errors
Lab products found in correlation
P stat5, by Cell Signaling Technology (1 mentions)
Rabbit antimurine collagen type 1 600 401 103 01, by Rockland Immunochemicals (1 mentions)
A5228, by Merck Group (1 mentions)
3 3 diaminobenzidine (dab), by Vector Laboratories (1 mentions)
Abc rtu, by Vector Laboratories (1 mentions)
Rat anti mouse cd31, by BD (1 mentions)
Bx51 fluorescence microscope, by Olympus (1 mentions)
3 protocols using rat antimouse f4 80 mca497r
1
Immunohistochemical Markers for Kidney Injury
2
Immunohistochemical Analysis of Tissue Markers
3
Fetal Liver Macrophage Characterization
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The fetal liver samples obtained from E14.5 mice (at least three samples per genotype) were fixed with 4% paraformaldehyde, included in an embedding medium [41 (link)] and cut (at 10 m) using a HM-560 cryomicrotome (Microm; Walldorf, Germany). Sections were incubated overnight with rat anti-mouse F4/80 (MCA497R; AbD Serotec, Oxford, UK) monoclonal antibody (1:200) revealed with a secondary antibody conjugated with cyanine 3.18 (1:300, 60 min, Jackson Immunoresearch Laboratories, West Grove, PA). Slides were observed and photographed using BX51 fluorescence microscope (Olympus, Milan, Italy) equipped with the Fuji S3 Pro digital camera (Fujifilm, Milan, Italy). Routine controls included substitution of primary antibody with PBS and/or dilution buffer only. Quantitation of macrophages was then carried out on five non-adjacent sections per samples and 4/5 fields per section.
Morphological examination was made in fetal liver sections (at least three samples for genotype) stained with hematoxylin/eosin.
Cytospins were prepared with 5 µl of fetal liver cells suspension (five mice per genotype, three slides per mouse) at a final concentration of 1 × 105 cell/µl. Slides were stained with Differential Quik Stain Kit (Modified Giemsa) (Polysciences, Inc.).
Morphological examination was made in fetal liver sections (at least three samples for genotype) stained with hematoxylin/eosin.
Cytospins were prepared with 5 µl of fetal liver cells suspension (five mice per genotype, three slides per mouse) at a final concentration of 1 × 105 cell/µl. Slides were stained with Differential Quik Stain Kit (Modified Giemsa) (Polysciences, Inc.).
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