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Secondary and isotype control antibodies

Manufactured by Dianova

Secondary and isotype-control antibodies are laboratory reagents used in immunoassays and cell-based experiments. They are designed to detect and quantify the presence of specific target molecules. These antibodies serve as tools to validate experimental results and ensure accurate data interpretation.

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2 protocols using secondary and isotype control antibodies

1

Comprehensive Chlamydia Antibody Panel

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Antibodies against chlamydia, chlamydial LPS, GAPDH, Atg7, Parkin, HDAC6, cPLA2 (phosphorylated and non-phosphorylated), H-2Kb, H-2Db, CD80, CD86, PD-L1, PD-L2, p38, P-p38, ERK1/2, P-ERK1/2, MAPKAPK-2, P-MAPKAPK-2, JNK, P-JNK, cJun, P-cJun, HSP25/27, P-HSP25/27, vimentin, Pink-1, p62, ubiquitin, acetylated α-tubulin, tubulin and β-actin were obtained from Sigma-Aldrich, Progen, Abcam, Upstate, eBioscience, CellSignaling, Thermo Scientific Pierce, antibodies-online and Millipore, respectively. Anti-Beclin-1, anti-LC3, and anti-chlamydial HSP60 were purchased from Abcam, Abgent Europe and Acris, respectively. Secondary and isotype-control antibodies were purchased from Dianova and BioLegend.
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2

Chlamydia Antibodies Immunodetection

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Antibodies against chlamydia (C. trachomatis, C. psittaci, and C. abortus), chlHSP60 (MAb A57-B9), cellular β-tubulin, and GAPDH, were obtained from Acris, Sigma-Aldrich, and Abcam. In addition, the fluorescein isothiocyanate (FITC)-conjugated mouse anti-chlamydia MAb (part of the IMAGEN Chlamydia Kit) was purchased from Oxoid. All of the secondary and isotype-control antibodies were purchased from Dianova and BioLegend.
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