Rabbit anti vapa

Manufactured by Proteintech

Rabbit anti‐VAPA is a polyclonal antibody produced in rabbits against the VAPA (Vesicle-associated membrane protein-associated protein A) protein. VAPA is an integral membrane protein involved in vesicle trafficking and membrane fusion processes. This antibody can be used to detect and study the VAPA protein in various applications.

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Lab products found in correlation

Mitotracker deep red fm, by Thermo Fisher Scientific (1 mentions) Paclitaxel, by Merck Group (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Mouse anti actin, by Proteintech (1 mentions) Rabbit anti myc, by Cell Signaling Technology (1 mentions) Lysotracker deep red, by Thermo Fisher Scientific (1 mentions) Wortmannin, by Thermo Fisher Scientific (1 mentions) Rapamycin, by Merck Group (1 mentions) Mouse anti myc, by Merck Group (1 mentions) Bafilomycin, by Merck Group (1 mentions) Rabbit anti ulk1, by Cell Signaling Technology (1 mentions) Ab105459, by Abcam (1 mentions) Ab117819, by Abcam (1 mentions) Mouse anti gfp, by Roche (1 mentions) Mouse anti lamp1, by BD (1 mentions) Rabbit anti fip200, by Merck Group (1 mentions) Sab4200135, by Merck Group (1 mentions) Rabbit anti atg13, by Cell Signaling Technology (1 mentions) Rabbit anti fip200, by Proteintech (1 mentions)

Close spelling variants of this product

Anti-TM (2 citations)

2 protocols using rabbit anti vapa

Fluorescent Labeling of Organelles

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Following antibodies were used in this study: Rabbit anti‐VAPA (Proteintech Cat#15275‐1‐AP), Rabbit anti‐VAPB (Proteintech Cat# 14477‐1‐AP), Rabbit anti‐KTN1 (Proteintech Cat#z19841‐1‐AP), Actin (Sigma‐Aldrich Cat# A5441), Anti‐Rabbit‐HRP (Thermo Fisher Cat#G‐21234), and Anti‐Mouse‐HRP (Thermo Fisher Cat#G‐21040). To inhibit microtubule (de)polymerization, tissue culture medium was supplemented with 2 µM of paclitaxel for two hours (Merck, USA) in DMSO. The late endosomal/lysosomal compartment was visualized using SiR‐lysosome at a concentration of 1 µM (Stein am Rhein, Switzerland). To ensure adequate retention of SiR‐lysosome, cells were treated with 10 µM Verapamil as per manufacturer’s recommendation. Mitochondria were labeled using Mitotracker Deep Red FM (Thermo Fischer, USA) at a concentration of 500 nM, as per manufacturers recommendation.

Spits M., Heesterbeek I.T., Voortman L.M., Akkermans J.J., Wijdeven R.H., Cabukusta B, & Neefjes J. (2021). Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture. EMBO Reports, 22(3), e50815.

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Autophagy Protein Immunodetection Assays

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The following antibodies were used for immunostaining or immunoblotting assays: mouse anti-LC3 (MBL, M152-3), rabbit anti-LC3 (2775S; Cell Signaling Technology), rabbit anti-p62 (MBL, PM045), rabbit anti-FIP200 (SAB4200135; Sigma-Aldrich), rabbit anti-FIP200 (17250-1-AP; Proteintech), rabbit anti-ULK1 (8054; Cell Signaling Technology), rabbit anti-Atg13 (13468S; Cell Signaling Technology), rabbit anti-ATL2 (16688-1-AP; Proteintech), rabbit anti-ATL3 (ab117819; Abcam), mouse anti-WIPI2 (ab105459; Abcam), mouse anti-VAPB (66191-1-IG; Proteintech), rabbit anti-VAPA (15275-1-AP; Proteintech), mouse anti-LAMP1 (553792; BD Biosciences), rabbit anti-PTPIP51 (20641-1-AP; Proteintech), rabbit anti-ATG101 (13492; Cell Signaling Technology), rabbit anti-Myc (2278S; Cell Signaling Technology), mouse anti-Myc (M5546; Sigma-Aldrich), mouse anti-GFP (11814460001; Roche), mouse anti-actin (60008-1-IG; Proteintech), and rabbit anti-HA (H6908; Sigma-Aldrich).
The following reagents were used in this study: wortmannin (PHZ1301; Life Technologies), bafilomycin (B1793; Sigma-Aldrich), rapamycin (R0395; Sigma-Aldrich), Lipofectamine 2000 (12566014; Life Technologies), Lipofectamine RNAi MAX (13778150; Life Technologies), and LysoTracker Deep Red (L12492; Thermo Fisher Scientific).

Liu N., Zhao H., Zhao Y.G., Hu J, & Zhang H. (2021). Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy. The Journal of Cell Biology, 220(7), e202012091.

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