Pregn 4 ene 3 20 dione

Phytosterol (total sterols content – 95.47%; β-sitosterol – 42.39%, stigmasterol – 26.08%, campesterol – 23.48%, brassicasterol – 3.52%) was obtained from Jiangsu Spring Fruit Biological Products Co., Ltd., China. Pregn-4-ene-3,20-dione (progesterone, Pr), pregn-4-en-17α-ol-3,20-dione (17α-hydroxy-progesterone, 17α-OH-Pr), pregn-4-ene-11α,17α-diol-3,20-dione (11α,17α-dihydroxy-progesterone, 11α,17α-di-OH-Pr) were purchased from Sigma (USA). 21-Acetoxy-pregn-4-ene-17α,21-diol-3,11,20-trione (21-acetate of cortisone, cortisone 21-acetate, AcC) was purchased from Steraloids (USA), yeast extract from Difco (USA), soya peptone from HiMedia (India), randomly methylated β-cyclodextrin (MCD) from Wacker Chemie (Germany). Other materials and solvents were of analytical grade and purchased from domestic commercial suppliers.

Cholesterol (cholest-5-en-3β-ol) was obtained from AppliChem (Germany); phytosterol (total sterols content—95.47%: β-sitosterol—42.39%, stigmasterol—26.08%, campesterol—23.48%, brassicasterol—3.52%)—from Jiangsu Spring Fruit Biological Products Co., Ltd. (China); pregn-4-ene-3,20-dione (progesterone); 3β-hydroxypregn-5-en-20-one (pregnenolone); 3β-hydroxyandrost-5-en-17-one (dehydroepiandrosterone, DHEA); acetamide—from Sigma-Aldrich (USA); yeast extract, bacto-peptone—from Difco (USA); randomly methylated β-cyclodextrin (MCD)—from Wacker-Chemie GmbH (Germany). Hygromycin B and DNA modifying enzymes were obtained from Thermo Fisher Scientific (USA); Tween 80—from Serva (Germany); DNA purification Kits—from Qiagen (Germany). The DNA manipulations were carried out according to the manufacturer’s instructions. All other reagents were of the highest purity grade and were purchased from domestic commercial suppliers (Russia).