Fluorescamine, Triton X-100, iodoacetamide (IAM) and all proteins including trypsin were obtained from Sigma-Aldrich (St. Louis, MO, USA). Hydrogen peroxide (H2O2), dimethyl sulfoxide (DMSO), Coomassie brilliant blue R-250, formic acid, acetonitrile (ACN), urea, dithiothreitol (DTT), anhydrous sodium phosphate dibasic, sodium chloride, ammonium bicarbonate, acrylamide, sodium dodecyl sulfate (SDS) and ammonium persulfate were purchased from Fisher Scientific (Waltham, MA, USA). The Laemmli sample buffer (2×) was obtained from Bio-Rad (Hercules, CA, USA). All proteins were purchased from Sigma-Aldrich (St. Louis, MO). Ultrapure water with electric resistance larger than 18.2 MΩ was produced by the Milli-Q water purification system (Millipore, Billerica, MA, USA).
Laemmli sample buffer 2
Manufactured by Bio-Rad
Sourced in United States
Laemmli sample buffer (2×) is a concentrated protein sample buffer used in gel electrophoresis to prepare protein samples for analysis. It is designed to denature proteins and equalize their charge-to-mass ratio, allowing for separation by size during electrophoresis.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (1 mentions)
Dithiothreitol (dtt), by Thermo Fisher Scientific (1 mentions)
Sodium dodecyl sulfate (sds), by Thermo Fisher Scientific (1 mentions)
Sodium chloride (nacl), by Thermo Fisher Scientific (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Merck Group (1 mentions)
Acrylamide, by Thermo Fisher Scientific (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Ammonium persulfate, by Thermo Fisher Scientific (1 mentions)
Ammonium bicarbonate, by Thermo Fisher Scientific (1 mentions)
Fluorescamine, by Merck Group (1 mentions)
Sodium phosphate dibasic anhydrous, by Thermo Fisher Scientific (1 mentions)
Bovine bile, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Alexa fluor 594 conjugated wheat germ agglutinin, by Thermo Fisher Scientific (1 mentions)
Iodoacetamide, by Merck Group (1 mentions)
4 protocols using laemmli sample buffer 2
1
Protein Assay and SDS-PAGE Protocol
2
Protein Extraction and Separation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Phosphate-buffered saline (PBS), paraformaldehyde, potassium chloride, sodium bicarbonate, sodium chloride, monopotassium phosphate, magnesium dichloride hexahydrate, ammonium carbonate, calcium chloride dihydrate, hydrochloric acid, bovine bile, porcine pepsin, porcine pancreatin and p-toluene-sulfonyl-L-arginine methyl ester (TAME) were obtained from Merck (Amsterdam, Netherlands). Laemmli sample buffer (2 ×) was obtained from Bio-Rad (Lunteren, The Netherlands). Non-heat inactivated non-irradiated fetal bovine calf serum(FBS) with product number FBS-12a was obtained from Capricorn Scientific (Ebsdorfergrund, Germany). Trifluoroacetic acid, dithiothreitol and iodoacetamide were obtained from Sigma Aldrich (Zwijndrecht, The Netherlands), and sequencing grade trypsin was obtained from Boehringer Mannheim (Mannheim, Germany). Precast SurePAGE MOPS-Tris PAGE gels were obtained from Genscript (Rijswijk, The Netherlands). Alexa-fluor 594-conjugated wheat germ agglutinin was obtained from Thermo Fisher (Bremen, Germany).
3
Comprehensive Immunoassay Reagent Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Phenol solution (90%), Quillaja Saponin (Catalog# S2149), silver nitrate, sodium carbonate, sodium bicarbonate, sodium acetate, Tween 20, DMSO, fetal bovine serum, and casein were obtained from Sigma Aldrich. Tamarind seed polymer (Xyloglucan, Glyloid 2A) was purchased from Sumitomo Pharma, Japan. Laemmli Sample Buffer (2×) and Precision Plus Protein Dual Color Standard were purchased from Bio-Rad. Trichloroacetic acid, 99%, Bovine Serum Albumin Standard Pre-Diluted Set, Coomassie R-250, and phosphate-buffered saline were purchased from ThermoFisher. TMB ELISA Substrate and TMB stop (Abcam, Cambridge, UK), cross-adsorbed goat anti-mouse IgA, IgG1, and IgG2a, HRP-conjugated (Invitrogen, Waltham, MA, USA), ExpressPlus PAGE Gel, 4–20% (Genscript, Piscataway, NJ, USA), Thiazolyl blue tetrazolium bromide (MTT, Trenton, NJ, USA), 98% (Acros Organics, Geel, Belgium) and Brucella Selective Supplement (Oxoid) were used in this study.
4
Whey Protein Isolate Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Whey protein isolate (WP, 94.7% protein content; 74.6% β-lactoglobulin, 23.8% α-lactalbumin, and 1.6% bovine serum albumin) was purchased from Davisco Food International Inc. (Le Sueur, MN, USA). Flaxseed (FLX) and sunflower (SO) oils were purchased in a local market. CO 2 ( purity, 99.995%) was purchased from Sapio (Monza, Italy). P 2 O 5 was purchased from Chem-Lab NV (Zedelgem, Belgium). Agar technical (Agar No. 3) was purchased from Oxoid Limited (Basingstoke, UK). Fast Green FCF and Nile Red dyes, porcine pepsin, porcine lipase, porcine pancreatin (8× USP), porcine bile extract, HCl, NaOH, CaCl 2 , Na 2 CO 3 , NaCl, KCl, KH 2 PO 4 , MgCl 2 (H 2 O) 6 , (NH 4 ) 2 CO 3 , MgSO 4 , Tris-HCl, SDS, bicinchoninic acid solution, cupric sulphate solution, acetic acid, Tris base, sodium dodecyl sulfate (SDS), glycine, and bovine serum albumin (BSA) were purchased from Sigma Aldrich (Milan, Italy). Absolute ethanol and methanol were purchased from J.T. Baker (Griesheim, Germany). Laemmli sample buffer 2×, β-mercaptoethanol, Mini-PROTEAN® TGX Stain-Free™ precast gels, Bio-Safe™ Coomassie G-250 stain, and the protein standards for SDS-PAGE (Precision Plus Protein Standards, Kaleidoscope) were purchased from Bio-Rad Laboratories Inc. (Hercules, California, USA). Deionized water (System Advantage A10®, Millipore S.A.S., Molsheim, France) was used for all the analyses.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!