Anti mouse cd41

Manufactured by BioLegend

Anti-mouse CD41 is a product used to detect the CD41 cell surface marker on mouse cells. CD41 is a platelet-specific integrin that plays a role in platelet activation and aggregation. This product can be used in flow cytometry and other immunoassays to identify and quantify CD41-expressing cells.

Automatically generated - may contain errors

Lab products found in correlation

Anti mouse cd31, by BioLegend (1 mentions) Anti mouse cd24, by BD (1 mentions) Anti mouse sca1, by BioLegend (1 mentions) Clone 36 e cadherin, by BD (1 mentions) Anti mouse ecadherin, by BD (1 mentions) Anti mouse cd45, by BioLegend (1 mentions) Hoechst 33342 trihydrochloride trihydrate, by Thermo Fisher Scientific (1 mentions) Whatman nucleopore track etch membranes, by Merck Group (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Allophycocyanin apc conjugated anti mouse rat cd62p, by BioLegend (1 mentions) Macsquant analyzer 10, by Miltenyi Biotec (1 mentions) Mouse anti cd31, by Santa Cruz Biotechnology (1 mentions) Endomucin v 7c7, by Santa Cruz Biotechnology (1 mentions) Cd45 30 f11, by BioLegend (1 mentions) Facsverse system, by BD (1 mentions)

4 protocols using anti mouse cd41

Endothelial Cell Marker Antibody Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

The antibodies used were as follows:
Anti-mouse Etv2 (Abcam, EPR5229(2); western blotting 1:100)
Anti-mouse Erg (Abcam, 9FY; western blotting 1:100)
Anti-mouse Fli1 (Abcam, ab15289; western blotting 1:100)
Anti-mouse CD31 (Biolegend clone 390; flow cytometry 1:2,000)
Anti-mouse VE-Cadherin (Biolegend clone BV13; flow cytometry 1:500)
Anti-mouse Vegfr2 (DC101; flow cytometry 1:500)
Anti-mouse CD62e (BD Biosceinces, clone 10E9.6; flow cytometry 1:500)
Anti-mouse Itgb1 (BD Biosciences, clone 18/CD29; flow cytometry 1:500)
Anti-mouse Meca32 (BD Biosciences, clone MECA-32; flow cytometry 1:500)
Anti-mouse Tie2 (Biolegend, clone Tek4; flow cytometry 1:500)
Anti-mouse ECadherin (BD Biosciences, Clone 36/E-Cadherin; flow cytometry 1:500)
Anti-mouse CD34 (BD Biosciences, Clone RAM34; flow cytometry 1:500)
Anti-mouse Vcam (BD Biosciences, Clone 429 MVCAM.A; flow cytometry 1:500)
Anti-mouse Sca1 (Biolegend, clone D7; flow cytometry 1:500)
Anti-mouse CD41 (Biolegend, clone MWReg30; flow cytometry 1:500)
Anti-mouse CD24 (BD Biosciences clone M1/69; flow cytometry 1:500)
Anti-mouse VE-Cadherin (R+D AF1002; IF 1:100)
Anti-mouse CD31 (Biocare, Clone Mec13.3; IF 1:100)
Uncropped western blotting images are shown in Supplementary Fig. 5.

Schachterle W., Badwe C.R., Palikuqi B., Kunar B., Ginsberg M., Lis R., Yokoyama M., Elemento O., Scandura J.M, & Rafii S. (2017). Sox17 drives functional engraftment of endothelium converted from non-vascular cells. Nature Communications, 8, 13963.

+ Open protocol

+ Expand

Fluorescent Liposome Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dextran 2000000 Da labeled with fluorescein isothiocyanate (FITC) was obtained from Sigma-Aldrich (St. Louis, MO). Egg phosphatidylcholine (EPC), hydrogenated soy phosphatidylcholine (HSPC), cholesterol, distearyl phosphatidylethanolamine (DSPE)-PEG-2000, DSPE-PEG-700, and DSPE-PEG-350 were obtained from Avanti Polar Lipids (Alabaster, AL,), DiR (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindotricarbocyanine iodide) and DiI (1,1′-dioctadecyl-3,3,3′3′-tetramethylindocarbocyanine perchlorate) were obtained from Biotium (Hayward, CA). Whatman Nucleopore Track-Etch Membranes (0.2 and 0.1 μm pore size) were obtained from Sigma-Aldrich (St. Louis, MO). Nitrocellulose membrane was obtained from Bio-Rad. FITC-dextran (2MDa) was obtained from Sigma. Hoechst 33342 trihydrochloride trihydrate was obtained from Thermo Fischer, Waltham, MA). PEGylated liposomal doxorubicin (LipoDox, Sun Pharma) was obtained from the University of Colorado hospital pharmacy and stored at 4 °C prior to use. Antimouse F/80, antimouse CD41, and antimouse CD45 antibodies were all from BioLegend (San Diego, CA). Secondary antibodies (AlexaFluor 488 labeled) were from Thermo Fisher.

Griffin J.I., Wang G., Smith W.J., Vu V.P., Scheinman R., Stitch D., Moldovan R., Moghimi S.M, & Simberg D. (2017). Revealing Dynamics of Accumulation of Systemically Injected Liposomes in the Skin by Intravital Microscopy. ACS nano, 11(11), 11584-11593.

+ Open protocol

+ Expand

Flow Cytometric Analysis of Platelet Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Freshly isolated or LPS stimulated platelets (10⁶) were resuspended in 50 μL of Tyrode's buffer. Platelets were incubated (RT for 30 min) with fluorescein isothocyanate (FITC) ‐conjugated anti‐mouse CD41 (Clone MWReg30, BioLegend, San Diego, CA) and Allophycocyanin (APC) conjugated anti‐mouse/rat CD62P (Clone RMP‐1, Biolegend). A minimum of 10,000 events per gate were acquired using a MACsQuant Analyzer 10 (Miltenyi Biotec, Auburn, CA) and analyzed using FlowLogic software (Innovai, Sydney, Australia). Platelets were distinguished by specific binding of anti‐CD41 and characteristic forward and side scattering. Platelets staining positive for CD41 and CD62P were designated as activated platelets.

Cornelius D.C., Baik C.H., Travis O.K., White D.L., Young C.M., Austin Pierce W., Shields C.A., Poudel B, & Williams J.M. (2019). NLRP3 inflammasome activation in platelets in response to sepsis. Physiological Reports, 7(9), e14073.

+ Open protocol

+ Expand

Bone Marrow Cell Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

BM MKs were stained with anti-mouse CD41, CD45 (30-F11; Biolegend) and CD61 (154-2C11; Biolegend) antibodies. BM ECs were stained with anti-mouse CD31 and endomucin (V.7C7; Santa Cruz) antibodies. The cell cycle and apoptosis were analyzed using a BD Biosciences system (San Jose, CA, USA) according to the kit instructions. After direct or indirect coculture, the growth of CD41^- CD45^- OBs was assessed as described previously 36 (link). Flow cytometric analysis was performed on a FACS-Verse system (BD Biosciences), and data were analyzed using FlowJo 10.3.0 (Tree Star, USA).

Tang Y., Hu M., Xu Y., Chen F., Chen S., Chen M., Qi Y., Shen M., Wang C., Lu Y., Zhang Z., Zeng H., Quan Y., Wang F., Su Y., Zeng D., Wang S, & Wang J. (2020). Megakaryocytes promote bone formation through coupling osteogenesis with angiogenesis by secreting TGF-β1. Theranostics, 10(5), 2229-2242.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!