Mouse vesiculin was synthesized in-house by the peptide synthesis group as per their published procedure in which the A- and B-peptide chains were first synthesized separately with protected cysteine residues before using regioselective disulfide bond formation;21 (link) stocks were prepared at a final concentration of 1 μg/μl in 16 mg/ml glycerol; 0.1% DDM. Insulin (Actrapid® Recombinant human-insulin 100 IU/ml; 3.47 μg/μl) was from Novo Nordisk (Bagsvaerd, Denmark). Both were diluted in physiological saline to the desired final concentrations. Blood glucose was measured in tail-vein blood (Advantage II; Roche). Core body temperature was recorded using a rectal probe (RET-3) coupled to a thermometer (Physitemp Instruments Inc).
Actrapid recombinant human insulin
Manufactured by Novo Nordisk
Sourced in Denmark, United Kingdom
Actrapid® Recombinant human-insulin is a laboratory product produced using recombinant DNA technology. It is a type of insulin, which is a hormone that regulates blood sugar levels. The core function of Actrapid® is to provide a standardized source of human insulin for research and laboratory applications.
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Lab products found in correlation
Advantage 2, by Roche (1 mentions)
Indinavir, by Merck Group (1 mentions)
Suramin, by Bio-Techne (1 mentions)
A438079, by Bio-Techne (1 mentions)
Mem amino acid solution, by Thermo Fisher Scientific (1 mentions)
Atpγs, by Bio-Techne (1 mentions)
5 bdbd, by Bio-Techne (1 mentions)
Fluo 4 direct, by Thermo Fisher Scientific (1 mentions)
Fatty acid free bsa, by Merck Group (1 mentions)
Ppads, by Bio-Techne (1 mentions)
Palmitic acid, by Merck Group (1 mentions)
2 protocols using actrapid recombinant human insulin
1
Peptide Synthesis and Characterization of Mouse Vesiculin
2
Recombinant Insulin Cell Treatments
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Novo Nordisk Actrapid recombinant human insulin was used for cell treatments (Henry Schein, United Kingdom). ATPγS, PPADs, suramin, 5-BDBD, A-438079 were purchased from Tocris (Bristol, United Kingdom). Fluo-4 Direct and 50 x MEM amino acid solution were from Thermo Fisher Scientific (Loughborough, United Kingdom). ATP (adenosine 5’-triphosphate magnesium salt), apyrase, fatty acid-free BSA, palmitic acid and indinavir were purchased from Sigma Aldrich (Poole, United Kingdom).
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