Invivo mab rat igg1 isotype control

Manufactured by BioXCell

Sourced in United States

The InVivo Mab rat IgG1 isotype control is a laboratory reagent used as a control in immunological experiments. It is an antibody of the IgG1 isotype derived from rat. This product serves as a reference to assist in the interpretation of experimental results involving antibody-based techniques.

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Lab products found in correlation

Acetyl coenzyme a assay kit, by Merck Group (1 mentions) Ecl reagent, by Thermo Fisher Scientific (1 mentions) Protein transport inhibitor cocktail, by BD (1 mentions) Acid phosphatase leukocyte trap kit 387a, by Merck Group (1 mentions) Foxp3 transcription factor staining buffer, by Thermo Fisher Scientific (1 mentions) Pe cy7 anti mouse cd5 53 7.3, by Thermo Fisher Scientific (1 mentions) Fitc anti mouse il 10 jes5 16e3, by BioLegend (1 mentions) Mouse tnf α 560478 elisa kit, by BD (1 mentions) Macrophage colony stimulating factor m csf 300 25, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Ionomycin, by Merck Group (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions)

2 protocols using invivo mab rat igg1 isotype control

Chromatin IP and Acetyl-CoA Assay

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Plasmids used in this study are described in Supplementary Table S3. Direct-Blot HRP anti-E. coli RNA Sigma 70 (SigA) antibody (663205) was purchased from BioLegend. Rabbit polyclonal to ESAT6 (ab45073) and polyclonal rabbit anti-Histone H3 (acetyl K9) antibody (ab4441, ChIP grade) were purchased from Abcam. The rabbit polyclonal antibody to FadA was generated by immunization of rabbits with the purified SUMO-FadA fusion protein, in collaboration with ABclonal Biotech, so as the isotype control antibody. InVivo Mab anti-mouse IL-6 (BE0046) and InVivo Mab rat IgG1 isotype control (BE0088) were purchased from BioXcell. Recombinant ESAT6 protein was from the laboratory stock. Enzymatic chromatin IP kit (magnetic beads) (9003) was from Cell Signaling Technology. Acetyl-Coenzyme A Assay Kit (MAK039) was from Sigma. ECL reagent (34075) was from Thermo.

Yang H., Wang F., Guo X., Liu F., Liu Z., Wu X., Zhao M., Ma M., Liu H., Qin L., Wang L., Tang T., Sha W., Wang Y., Chen J., Huang X., Wang J., Peng C., Zheng R., Tang F., Zhang L., Wu C., Oehlers S.H., Song Z., She J., Feng H., Xie X, & Ge B. (2021). Interception of host fatty acid metabolism by mycobacteria under hypoxia to suppress anti-TB immunity. Cell Discovery, 7, 90.

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Murine Immune Cell Identification

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The following antibodies/kits were obtained from eBiosciences (USA): PerCp-Cy5.5 Anti-Mouse-CD19 (1D3) (45-0193-82), PE-Cy7 Anti-Mouse-CD5 (53-7.3) (25-0051-81), APC Anti-Mouse-CD1d (1B1) (17-0011-82), Foxp3/Transcription factor staining buffer (0-5523-00), and RBC lysis buffer (00-4300-54). FITC Anti-Mouse-IL-10 (JES5-16E3) (505005) was purchased from Biolegend (USA). InVivoMAb anti-mouse IL-10 antibody (BE0049) and InVivoMAb Rat IgG1 isotype control were purchased from BioXcell (USA). Protein transport inhibitor cocktail and the Mouse TNF-α (560478) ELISA kit were procured from BD (USA). The following ELISA kits were brought from R&D and Elabscience: Mouse IL-10 (M1000B) (E-EL-M0046) and Mouse IL-17 (M1700). PMA, Ionomycin, LPS (Escherichia coli serotype 0111: B4), FITC-Phalloidin (P5282), DAPI, and Acid phosphatase leukocyte (TRAP) kit (387A) were procured from Sigma-Aldrich (USA). Macrophage-colony stimulating factor (M-CSF) (300-25) and Receptor activator of nuclear factor κB-ligand (sRANKL) (310-01) were procured from PeproTech (USA). α-Minimal essential media (MEM) and RPMI-1640 were obtained from Gibco (Thermo Fisher Scientific, USA).

Sapra L., Bhardwaj A., Mishra P.K., Garg B., Verma B., Mishra G.C, & Srivastava R.K. (2021). Regulatory B Cells (Bregs) Inhibit Osteoclastogenesis and Play a Potential Role in Ameliorating Ovariectomy-Induced Bone Loss. Frontiers in Immunology, 12, 691081.

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