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Eclipse ni e epifluorescence microscope

Manufactured by Nikon
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The Eclipse Ni-E epifluorescence microscope is a laboratory equipment product manufactured by Nikon. It is designed for fluorescence microscopy applications, providing the capability to observe and analyze samples using fluorescent labeling techniques.

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Lab products found in correlation

Vectashield hardset mounting medium with dapi, by Vector Laboratories (2 mentions) Bodipy 493 503 dye, by Thermo Fisher Scientific (1 mentions) Cm3050, by Leica camera (1 mentions)

6 protocols using eclipse ni e epifluorescence microscope

1

Comet Assay for DNA Damage

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Comet assays were performed using CometAssay® Trevigen Kit (4250-050-K) following manufacturer’s instructions. Pictures were taken using a Nikon Eclipse Ni-E epifluorescence microscope and tail moment was calculated using the OPENCOMET plugin for Fiji.
Colomer C., Margalef P., Villanueva A., Vert A., Pecharroman I., Solé L., González-Farré M., Alonso J., Montagut C., Martinez-Iniesta M., Bertran J., Borràs E., Iglesias M., Sabidó E., Bigas A., Boulton S.J, & Espinosa L. (2019). IKKα Kinase Regulates the DNA Damage Response and Drives Chemo-resistance in Cancer. Molecular Cell, 75(4), 669-682.e5.
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2

Brain Tissue Preparation for Imaging

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Mice were perfused transcardially with 10% phosphate buffered saline until outflow ran clear and then with 10% phosphate buffered formalin. Brains were then extracted and post-fixed in formalin for 2–4 days, and then transferred to 30% sucrose solution in phosphate buffered saline for 1–2 days. Brains were then frozen and sliced into 40 um sections on a cryostat (Leica CM 3050S), mounted, and coverslipped with Vectashield Hardset mounting medium with DAPI (Vector Laboratories). Slides were then imaged using a Nikon Eclipse Ni-E epifluorescence microscope at 10x and 20x to verify viral expression and location and GRIN lens location relative to the CA1 cell layer.
Levy S.J., Kinsky N.R., Mau W., Sullivan D.W, & Hasselmo M.E. (2020). Hippocampal spatial memory representations in mice are heterogeneously stable. Hippocampus, 31(3), 244-260.
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3

Comet Assay for DNA Damage

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Comet assays were performed using Comet Assay Kit following manufacturer’s instructions. Pictures were taken using a Nikon Eclipse Ni-E epifluorescence microscope and tail moment was calculated using the OPENCOMET plugin for Fiji.
Solé L., Lobo-Jarne T., Álvarez-Villanueva D., Alonso-Marañón J., Guillén Y., Guix M., Sangrador I., Rozalén C., Vert A., Barbachano A., Lop J., Salido M., Bellosillo B., García-Romero R., Garrido M., González J., Martínez-Iniesta M., López-Arribillaga E., Salazar R., Montagut C., Torres F., Iglesias M., Celià-Terrassa T., Muñoz A., Villanueva A., Bigas A, & Espinosa L. (2022). p53 wild-type colorectal cancer cells that express a fetal gene signature are associated with metastasis and poor prognosis. Nature Communications, 13, 2866.
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4

Visualization of PLA Accumulation

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To observe PLA accumulation, cells were grown as described in section “Culture Conditions for PLA Production” and washed twice with water. Pellets were then resuspended in water and PLA accumulation was immediately visualized in the cells using BODIPY 493/503 dye (Thermo Fisher Scientific) diluted at 1 μg/mL and imaged using Eclipse Ni-E epifluorescence microscope (Nikon France S.A, Champigny sur Marne, France).
Lajus S., Dusséaux S., Verbeke J., Rigouin C., Guo Z., Fatarova M., Bellvert F., Borsenberger V., Bressy M., Nicaud J.M., Marty A, & Bordes F. (2020). Engineering the Yeast Yarrowia lipolytica for Production of Polylactic Acid Homopolymer. Frontiers in Bioengineering and Biotechnology, 8, 954.
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5

Comet Assay for DNA Damage Assessment

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HT29-M6 cells were plated in 12-well plates with Dulbecco’s modified Eagle’s medium. 4 days after arriving to 100% confluency, cells were treated with 5-FU + iri. (25 µg/mL 5-Fu + 10 µg/mL irinotecan) alone or in combination with secretion inhibitor benzamil 20 µM for 72 hr. Comet assays were performed using CometAssay Trevigen Kit (4250-050K) following the manufacturer’s instructions. Pictures were taken using a Nikon Eclipse Ni-E epifluorescence microscope, and tail moment was calculated using the OPENCOMET plugin for ImageJ.
Cantero-Recasens G., Alonso-Marañón J., Lobo-Jarne T., Garrido M., Iglesias M., Espinosa L, & Malhotra V. (2022). Reversing chemorefraction in colorectal cancer cells by controlling mucin secretion. eLife, 11, e73926.
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6

Tissue Preparation for Fluorescent Imaging

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Mice were perfused transcardially with 10% phosphate buffered saline (KPBS) followed by formalin. Brains were then extracted and post-fixed in formalin for 2–4 additional days, and were transferred to a 30% sucrose solution in KPBS for 1–2 additional days. Brains were then frozen and sliced in 40 μm sections on a cryostat (Leica CM 3050S), mounted, and cover slipped with Vectashield Hardset mounting medium with DAPI (Vector Laboratories). Slides were then imaged using a Nikon Eclipse Ni-E epifluorescence microscope at 4x, 10x, and 20x to verify viral expression levels, location, and GRIN lens placement above the CA1 cell layer.
Kinsky N.R., Sullivan D.W., Mau W., Hasselmo M.E, & Eichenbaum H.B. (2018). Hippocampal Place Fields Maintain a Coherent and Flexible Map Across Long Time Scales. Current biology : CB, 28(22), 3578-3588.e6.
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