To investigate the possible signaling pathway involved in the induction of COL1A1 expression by overexpression of both hTNX-FGFFFF and integrin α11 in LX-2 cells, a TGF-β receptor type 1 (TGFBRI) inhibitor (SB525334) (MedChemExpress) and a YAP inhibitor (verteporfin) (Cayman Chemical) were used. Each of the inhibitors was dissolved in dimethyl sulfoxide (DMSO). After transfection as described above, LX-2 cells were cultured in DMEM with 0.5% FBS for 24 h, and then the culture was further continued with 2 µM verteporfin for 19 h or with 10 µM SB525334 for 42 h. The inhibitor-treated LX-2 cells were collected and then RNA was extracted from the cells.
Sb525334
Manufactured by MedChemExpress
SB525334 is a small molecule compound used as a laboratory reagent. It is a selective and potent inhibitor of protein kinases. This product is intended for research use only and not for use in humans or animals.
Automatically generated - may contain errors
Lab products found in correlation
Verteporfin, by Cayman Chemical (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Pifithrin α, by MedChemExpress (1 mentions)
F12 medium, by Thermo Fisher Scientific (1 mentions)
Tgf β1, by Merck Group (1 mentions)
Hk 2 cell, by American Type Culture Collection (1 mentions)
Erlotinib, by LC Laboratories (1 mentions)
Matrigel, by BD (1 mentions)
Stereotaxic instrument, by Stoelting (1 mentions)
3 protocols using sb525334
1
Investigating COL1A1 Induction Signaling
2
Pifithrin-α, SB525334 and ISL Effects on HK-2 Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HK-2 cells were purchased from the American Type Culture Collection (ATCC) and maintained in F12 medium (GIBCO) with 10% FBS (GIBCO) at 37°C in a 5% CO2 humidified incubator.
Pifithrin-α, SB525334, and ISL were purchased from MedChemExpress, reconstituted in DMSO, and used at a final concentration of 20, 1, and 20 μM, respectively. TGF-β1 was purchased from Sigma-Aldrich, reconstituted in PBS and used at a final concentration of 10 ng/ml.
Pifithrin-α, SB525334, and ISL were purchased from MedChemExpress, reconstituted in DMSO, and used at a final concentration of 20, 1, and 20 μM, respectively. TGF-β1 was purchased from Sigma-Aldrich, reconstituted in PBS and used at a final concentration of 10 ng/ml.
3
Orthotopic and Subcutaneous Glioma Mouse Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Low passage iEIP or patient-derived glioma cells were retrovirally transduced with luciferase cassette. 8-to 12-week-old RAG1 À/À mice were anesthetized and restrained using a stereotaxic instrument (Stoelting). 50,000 iEIP glioma cells in 2 mL PBS were injected into right caudate nucleus 2.2 mm below the surface of the brain at 1-mm anterior and 1-mm lateral from the bregma. For subcutaneous grafting, iEIP glioma cells were resuspended in 50% Matrigel (BD Biosciences; #356231) in PBS and approximately 10,000,000 cells were injected into each flank of Rag1 À/À mice. Doxycycline (Research Product International Inc. #D43020) was administered as 2 g/L with 5% sucrose in drinking water. Erlotinib (LC Laboratories, #E4007) was administered as 150 mg/kg/d by gavage. SB525334 (medchemexpress, #HY-12043) was treated as 10 mg/kg/d by intraperitoneal injection. Tumor growth was monitored and measured every 7 days by caliper, and volume was calculated by the formula:
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!