Phosphodiesterase 2 from bovine spleen

Manufactured by Merck Group

Phosphodiesterase II is an enzyme isolated from bovine spleen. Its core function is the hydrolysis of cyclic nucleotides, specifically the conversion of cyclic AMP and cyclic GMP to their respective 5'-monophosphates.

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Lab products found in correlation

Rnase a, by Qiagen (1 mentions) Proteinase k, by Qiagen (1 mentions) Calf thymus dna, by Worthington (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions) Zinc powder, by Merck Group (1 mentions) Acetonitrile acn ulc ms grade, by Biosolve (1 mentions) Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) Trypsin edta, by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Alkaline phosphatase, by Merck Group (1 mentions) Fetal calf serum (fcs), by Lonza (1 mentions)

2 protocols using phosphodiesterase 2 from bovine spleen

Synthesis and Purification of DNA Standards

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(R)-NNN, (S)-NNN and racemic NNN were synthesized as described previously.^{19 , 20} POB-DNA standards and deuterium labeled internal standards were synthesized previously in our laboratory.^{17 (link), 21 (link)–23 (link)} Puregene DNA purification solutions, proteinase K and RNase A were purchased from Qiagen (Valencia, CA). Calf thymus DNA and micrococcal nuclease (from Staphylococcus aureus) were from Worthington Biochemical (Lakewood, NJ). Phosphodiesterase II (from bovine spleen) was purchased from Sigma-Aldrich (St. Louis, MO). Alkaline phosphatase (from calf intestine) was obtained from Roche Diagnostics (Indianapolis, IN). All other chemicals were from Sigma-Aldrich or Fisher Scientific (Fairlawn, NJ).

Yang J., Villalta P.W., Upadhyaya P, & Hecht S.S. (2015). Analysis of O6-[4-(3-Pyridyl)-4-oxobut-1-yl]-2′-deoxyguanosine and Other DNA Adducts in Rats Treated with Enantiomeric or Racemic N′-Nitrosonornicotine. Chemical research in toxicology, 29(1), 87-95.

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Analysis of DNA Oxidation Markers

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AAI was purchased from Sigma‐Aldrich. The LLC‐PK1 porcine cell line (ATCC® CL‐101™) was obtained from the American Type Culture Collection (ATCC). Dulbecco's modified Eagle medium (DMEM), phosphate‐buffered saline and trypsin‐EDTA were purchased from Gibco and fetal calf serum from Lonza BioWhittaker. dA, deoxyguanosine (dG), N,N‐dimethylformamide, zinc powder, phosphodiesterase I from Crotalus adamanteus (venom phosphodiesterase), phosphodiesterase II from bovine spleen (spleen phosphodiesterase), nucleus PI and alkaline phosphatase were purchased from Sigma‐Aldrich. Dimethyl sulfoxide (>99.9%) was obtained from Acros Organics. Acetonitrile (ACN; ULC/MS grade) was obtained from Biosolve BV. Formic acid and ethanol were obtained from VWR Merck.

Abdullah R., Wesseling S., Spenkelink B., Louisse J., Punt A, & Rietjens I.M. (2020). Defining in vivo dose‐response curves for kidney DNA adduct formation of aristolochic acid I in rat, mouse and human by an in vitro and physiologically based kinetic modeling approach. Journal of Applied Toxicology, 40(12), 1647-1660.

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