Cd206 antibody

Manufactured by Thermo Fisher Scientific

Sourced in United States

The CD206 antibody is a laboratory reagent used in flow cytometry and immunohistochemistry applications. It binds to the CD206 receptor, which is expressed on the surface of certain immune cells. The CD206 antibody can be used to identify and quantify these cell populations in research samples.

Automatically generated - may contain errors

Lab products found in correlation

F 13838, by Thermo Fisher Scientific (1 mentions) Cytoflex, by Beckman Coulter (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions) Mouse igg1 kappa, by Thermo Fisher Scientific (1 mentions) Flow cytometry, by Beckman Coulter (1 mentions)

6 protocols using cd206 antibody

Characterization of apoEV Size and Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The intensity of the forward scatter (FSC) signal is proportional to the particle size. Thus, the size of apoEVs was analyzed using the FSC signal by comparison with that of a population of microsphere standards (Thermo Fisher, F-13838, USA) with known diameters (1 μm, 2 μm, 4 μm, and 6 μm).
Annexin V apoptosis detection kits (Beyotime, C1077, China) were used to mark apoptotic cells and apoEVs. For cell biomarker analysis, cells were fixed with 4 % paraformaldehyde and permeabilized with saponin. Then, the fixed cells were incubated with antibodies in 5 % BSA. After washing with PBS, the stained cells were analyzed with flow cytometry (BD, LSRFortessa™, USA). The antibodies used in this study were CD206 antibody (Thermo Fisher, 17-2061-80, USA) and CCR7 antibody (Thermo Fisher, 12-1971-82, USA).

Li X., Li S., Fu X, & Wang Y. (2024). Apoptotic extracellular vesicles restore homeostasis of the articular microenvironment for the treatment of rheumatoid arthritis. Bioactive Materials, 35, 564-576.

+ Open protocol

+ Expand

Quantifying Osteoclast Marker Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell suspensions containing approximately 1 × 10⁶ cells was centrifuged at 400 × g for 5 min, resuspended in 100 µL FACS (PBS with 2% FBS) and incubated with 2 µL additional Fc blocking agent (BD Biosciences, USA) at 4 °C for 5 min. After a further incubation with CD206 antibody (ThermoFisher, USA) and CD61 antibody (a marker of osteoclasts) (ThermoFisher, USA) on ice for 20–30 min, samples were then centrifuged (400 × g for 5 min) and resuspended in 400 uL FACS for flow cytometry. Signals were detected with a flow cytometer (CytoFLEX, Beckman Coulter), on‐board assays were performed and the data were analyzed with FlowJo software.

Li D., Jiang Y., He P., Li Y., Wu Y., Lei W., Liu N., de Bruijn J.D., Zhang H., Zhang H., Ji P., Yuan H, & Li M. (2023). Hypoxia Drives Material‐Induced Heterotopic Bone Formation by Enhancing Osteoclastogenesis via M2/Lipid‐Loaded Macrophage Axis. Advanced Science, 10(15), 2207224.

+ Open protocol

+ Expand

Evaluating Angiogenesis and Macrophage Phenotypes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence staining of CD31 provides a promising evaluation of the formation of new capillaries in the tissue and the rate of deposition of new bone [50 (link)]. Briefly, sections were incubated overnight at 4°C with a primary antibody (1:200, Affinity, AF61911) followed by incubation with Cy3-coupled goat anti-rabbit secondary antibody (1:200, Affinity, S0011) for 1 h at 37°C. Finally, the nuclei were stained with DAPI (Solarbio, China). Images were captured with a fluorescence microscope and analyzed using Image-Pro Plus software (version 7.0).
The percentage of macrophages with different phenotypes and differentiation was determined. Briefly, M1-type macrophages were labeled with a CCR7 antibody (1:100, Affinity, AF5293) and incubated overnight at 4°C with the Alexa 488 donkey anti-rabbit IgG secondary antibody (1:200, Yeasen, 34206ES60, China). Likewise, M2 macrophages were labeled with CD206 antibody (1:100, Thermo Fisher, PA5-46994). The nuclei were stained with DAPI. The images by fluorescent microscopy were captured and analyzed with Image-Pro Plus software (version 7.0).

Zhou P., Yan B., Wei B., Fu L., Wang Y., Wang W., Zhang L, & Mao Y. (2023). Quercetin-solid lipid nanoparticle-embedded hyaluronic acid functionalized hydrogel for immunomodulation to promote bone reconstruction. Regenerative Biomaterials, 10, rbad025.

+ Open protocol

+ Expand

Murine BMDM Phenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

After the aforementioned treatment, murine BMDM were digested and washed with PBS three times. Cells were collected and incubated with CD68 antibody (1:500, eBioscience, San Diego, CA, USA) or CD206 antibody (1:500, eBioscience, San Diego, CA, USA) for 30 min at room temperature. Mouse IgG1 kappa was used as negative control (eBioscience, San Diego, CA, USA). The ratio of CD68⁺ cells and CD206⁺ cells were analyzed using a flow cytometer (Thermo Fisher Scientific, Waltham, MA, USA).

Wang S.L., Shao B.Z., Zhao S.B., Chang X., Wang P., Miao C.Y., Li Z.S, & Bai Y. (2019). Intestinal autophagy links psychosocial stress with gut microbiota to promote inflammatory bowel disease. Cell Death & Disease, 10(6), 391.

+ Open protocol

+ Expand

Phenotypic Profiling of Murine Macrophages

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

In a 12-well plate, 1 × 10⁵ murine-derived macrophage cells (RAW264.7) were seeded per well. After culturing for 4 days, the cells were digested, centrifuged, and washed with 1% BSA for 0.5 h to block non-specific antigens. Cells were then stained with fluorescent-conjugated CD68 and CD86 antibodies (eBioscience, USA) as M1 markers and CD206 antibody (eBioscience, USA) as M2 marker for 1 h at room temperature [34 (link)]. After washing the cells three times with 1% BSA, 100 μl of the suspension was aspirated into a 96-well plate for analysis by flow cytometry (LSRFortessa, BD, USA).

Li C., Sun F., Tian J., Li J., Sun H., Zhang Y., Guo S., Lin Y., Sun X, & Zhao Y. (2022). Continuously released Zn2+ in 3D-printed PLGA/β-TCP/Zn scaffolds for bone defect repair by improving osteoinductive and anti-inflammatory properties. Bioactive Materials, 24, 361-375.

+ Open protocol

+ Expand

Flow Cytometry Analysis of CD86 and CD206

Check if the same lab product or an alternative is used in the 5 most similar protocols

After resuspending the cells, CD86 antibody (eBioscience, San Diego, 12-0862-82, USA) and CD206 antibody (eBioscience, 12-0114-82, USA) were added and incubated with the cells in the dark for 30 min. The cells were subsequently resuspended with PBS, filtered through a nylon mesh, and analyzed by a flow cytometry (Beckman, USA).

Wu R., Zhou T., Xiong J., Zhang Z., Tian S., Wang Y., Chen J, & Tian X. (2022). Quercetin, the Ingredient of Xihuang Pills, Inhibits Hepatocellular Carcinoma by Regulating Autophagy and Macrophage Polarization. Frontiers in bioscience (Landmark edition), 27(12).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!