All animal experimental procedures were approved by the Ethics Committee of the Shenzhen TopBiotech Co. Ltd (NO. TOP-IACUC-2021-0129). Healthy adult male KM mice weighing between 20 and 25 g were purchased from the Shenzhen TopBiotech Co. Ltd (Shenzhen, China). All mice were housed in specific pathogen-free (SPF) facilities, fed standard rodent chow and water, and maintained at a controlled temperature (25 ± 2°C) under a light cycle (12 h light/12 h dark). AP model was induced by intraperitoneal injection of caerulein (50 μg/kg, MedChemExpress, USA) seven times at an interval of 1 h. Vehicle or PSI-697(30 mg/kg, intragastric administration, MedChemExpress, USA) was given 1 h before the first injection of caerulein. Animals were sacrificed 18 h after induction of AP and blood and pancreatic samples were collected. Blood were centrifuged at 4°C, 3000 rpm for 10 min to obtain serum. Serum amylase and lipase levels were detected by the use of a VITROS 5.1 FS automatic biochemistry analyzer (Johnson & Johnson).
Caerulein
Manufactured by MedChemExpress
Sourced in United States
Caerulein is a synthetic peptide that mimics the biological effects of the natural hormone cholecystokinin (CCK). It is primarily used as a laboratory tool for scientific research and experiments.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using caerulein
1
Caerulein-Induced Acute Pancreatitis in Mice
2
Emodin Modulates ARJ21 Cells
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Emodin was purchased from Beijing Solarbio Science & Technology Co., Ltd. IL-2 (cat. no. SRP3242) and anti-CD3 (cat. no. SAB4700040)/CD28 (cat. no. SAB4700739) were purchased from Sigma-Aldrich; Merck KGaA. Caerulein was obtained from MedChemExpress. Meanwhile, ARJ21 cells were treated with 20 and 40 µM Emodin, according to previous references (28 (link),29 (link)).
3
Caerulein-Induced Chronic Pancreatitis in Mice
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Chronic pancreatitis was induced using established protocols (31 (link)). Caerulein (MedChemExpress) was reconstituted in sterile phosphate-buffered saline. Adult mice (6 to 10 weeks old) were injected with Caerulein (individual dose, 50 μg/kg), given as four hourly intraperitoneal injections, three times per week for three consecutive weeks.
4
Caerulein-Induced Acute Pancreatitis Model
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Purple speckled kidney beans (Phaseolus vulgaris) were provided by the reclamation area of Heihe (China). Male Wistar rats (SPF, Permit number: SCXK (Liao) 2020-0001) were purchased from Changsheng Biotechnology Co., Ltd. (Benxi, China). Caerulein (≥99%) was obtained from Med Chem Express (Monmouth Junction, NJ, USA). Octreotide acetate injection was from Novartis Pharmaceutical Co., Ltd. (Beijing, China). Assay kits for tumor necrosis factor-α (TNF-α), interleukine-1 beta (IL-1β), interleukine-6 (IL-6), diamine oxidase (DAO), endotoxin (ET), and D-lactic acid (DLA) were provided by Calvin Biotechnology Co., Ltd. (Suzhou, China). Assay kits for triglyceride (TG), total cholesterol (TC), and myeloperoxidase (MPO) were purchased from the Institute of Bioengineering (Nanjing, China). The NCBI primer designing tool designed RT-PCR amplification primers, and the primer sequence was synthesized by Shenggong Biotechnology Co., Ltd. (Shanghai, China).
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