Ovarian cancer cells (OVCAR-3, A2780/CP70) and normal ovarian cells (IOSE 364) were seeded in 96-well plates at a density of 1 × 104/well (medium RPM-1640 + 10% FBS) and incubated at 37 °C for 16 h. Then, the culture medium was removed and cells incubated with different concentrations of CHSP (5–40 μg/mL) or DMSO (as vehicle) for 24 h. After treatment, the cells were washed twice with phosphate-buffered saline (PBS), introduced to 100 μL freshly prepared Aqueous One Solution (MTS tetrazolium compound) (Promega, Madison, WI, USA) in medium, and incubated for 1 h at 37 °C. Cells were then transferred to a microplate reader and the absorption peak was checked at 490 nm. Cell viability was expressed as a percentage of the control.
Aqueous one solution mts tetrazolium compound
Manufactured by Promega
Sourced in United States
Aqueous One Solution (MTS tetrazolium compound) is a colorimetric assay reagent used for the quantification of cell proliferation, viability and cytotoxicity in cell-based assays. The reagent contains a tetrazolium compound that is bio-reduced by viable cells, producing a colored formazan product that can be measured spectrophotometrically.
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3 protocols using aqueous one solution mts tetrazolium compound
1
Evaluating Ovarian Cancer Cell Viability
2
Measuring Cell Viability via MTS Assay
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To measure cell viability, cells were seeded in 96-well plates at a density of 1×104/well and allowed to recover, attach to the substrate, and grown to log phase overnight. After incubation 37°C, the culture medium was removed and incubated with different concentrations of GA for 24 h. Each treatment was performed in triplicate. After treatment, the cells were washed twice with phosphate-buffered saline (PBS), introduced to 100 μl freshly prepared AQueous One Solution (MTS tetrazolium compound) (Promega, Madison, WI, USA) in medium, and incubated for 1 h at 37°C. The OD values, at 490 nm, were measured by an enzyme-linked immunosorbent assay (ELISA) plate reader (Bio-Tek Instruments, Winooski, VT, USA). Cell viability was expressed as a percentage of the control.
3
Evaluating Ovarian Cancer Cell Viability
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