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Sulfo nhs xbiotin

Manufactured by Thermo Fisher Scientific

Sulfo-NHS-Xbiotin is a water-soluble biotin derivative used for labeling proteins. It contains a sulfo-N-hydroxysuccinimide (sulfo-NHS) ester group that can form covalent bonds with primary amine groups on proteins. The biotin moiety allows for detection or purification of labeled proteins using streptavidin-based systems.

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2 protocols using sulfo nhs xbiotin

1

Virus-like Particle ELISA Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Virus-like particles containing protease-purified Env trimers were prepared as previously described50 (link),51 (link),91 (link), and ELISAs on these VLPs were performed as described51 (link). Briefly, Immulon II plates were coated overnight at 4°C with VLPs at 20 times their concentration in transfection supernatants. Wells were washed with PBS and then blocked with 4% bovine serum albumin/10% fetal bovine serum in PBS. Various biotinylated monoclonal antibodies (biotinylated using sulfo-NHS-Xbiotin, Thermo), and CD4-IgG2 were then titrated in the presence or absence of a fixed concentration of 2μg/ml soluble CD4. Alkaline phosphatase conjugated to streptavidin (Vector Laboratories, Burlingame, CA; to detect biotinylated mAbs) or anti-Fc (Accurate, Westbury, NY; to detect CD4-IgG2) and SigmaFAST p-nitrophenyl phosphate tablets (Sigma) were then used to detect binding. Plates were read at 405 nm.
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2

Virus-like Particle ELISA Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Virus-like particles containing protease-purified Env trimers were prepared as previously described50 (link),51 (link),91 (link), and ELISAs on these VLPs were performed as described51 (link). Briefly, Immulon II plates were coated overnight at 4°C with VLPs at 20 times their concentration in transfection supernatants. Wells were washed with PBS and then blocked with 4% bovine serum albumin/10% fetal bovine serum in PBS. Various biotinylated monoclonal antibodies (biotinylated using sulfo-NHS-Xbiotin, Thermo), and CD4-IgG2 were then titrated in the presence or absence of a fixed concentration of 2μg/ml soluble CD4. Alkaline phosphatase conjugated to streptavidin (Vector Laboratories, Burlingame, CA; to detect biotinylated mAbs) or anti-Fc (Accurate, Westbury, NY; to detect CD4-IgG2) and SigmaFAST p-nitrophenyl phosphate tablets (Sigma) were then used to detect binding. Plates were read at 405 nm.
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