Anti thbs2

Protein concentrations from the cell lysates were measured as described above by BCA assay and 20μg of protein were resolved in SDS-PAGE gels and transferred to 0.2μm nitrocellulose membranes. 12% and 6% gels were used for separation of lower and higher molecular weight proteins, respectively. Rabbit anti-PSMA2 (Cell Signaling, Cat. 2455), anti-THBS2 (Abcam, Cat. ab84469), and anti–CST3 (Abcam, Cat. Ab109508), and mouse anti-ZIKV NS1 (BioFront Technologies Cat. BF-1225-06), anti-caspase 3 (Cell Signaling Cat. 3G2), anti-STAT1 (Cell Signaling, Cat. 9176S) and anti-Beta-Actin (Cell Signaling, Cat. 3700S) primary antibodies were used to detect specific proteins. (HRP)-conjugated horse anti-mouse (Cell Signaling, Cat. #7076) or anti-rabbit (Cell Signaling, Cat. #7074) secondary antibodies were used for detection of the immune complexes. Protein bands were imaged with an Alpha Innotech FluorChemQ MultiImage III after developed with ECL reagents. Band intensities were quantified with Image J 1.50i (USA) and graphically presented by Graphpad Prism software (La Jolla, California, USA).

The following antibodies were used in the study: anti‐SFRP2 (Santa Cruz, sc‐365,524, 1:300), anti‐THBS2 (Abcam, ab84469, 1:500), anti‐HSP90 (ABclonal, A5027, 1:2000) and anti‐GAPDH (ZSGB‐BIO, TA‐08, 1:2000). Secondary antibodies (Jackson Immuno Research Laboratories, West Grove, PA, USA) were diluted 1:4000 using 5% skim milk in Tris‐buffered saline.