IL was purchased from the National Institute for Food and Drug Control (Beijing, China). MTT was purchased from BioFroxx (Darmstadt, Germany). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, USA). Crystal violet was purchased from Solarbio (Beijing, China). Hoechst 33258 was purchased from Sigma-Aldrich (St Louis, USA). DME/F12 medium and RPMI 1640 medium were purchased from HyClone Laboratories (Logan, USA). GSH and oxidized glutathione disulfide (GSSG) assay kit was purchased from Beyotime Biotechnology (Shanghai, China). Primary antibodies against Bax (#50599-2-lg, 1:1000), p53 (#10442-1-AP, 1:1000), GAPDH (#10494-1-AP, 1:6500), PARP (#13371-1-AP, 1:500), and horseradish peroxidase (HRP)-conjugated secondary antibody were purchased from ProteinTech Group (Rosemont, USA). Primary antibodies against Akt (#9272, 1:2000), anti-phospho-Akt (Ser473) (#9271, 1:2000), Bcl-2 (#2876, 1:1000) and caspase-3 (#9662, 1:1000) were purchased from Cell Signaling Technology (Danvers, USA). Annexin V-FITC/PI Apoptosis Detection Kit was purchased from CoWin Biosciences (Beijing, China).
Parp 13371 1 ap
Manufactured by Proteintech
Sourced in United States
PARP (#13371-1-AP) is a primary antibody that targets Poly(ADP-ribose) polymerase, a nuclear enzyme involved in DNA repair processes. The antibody is suitable for applications such as Western Blotting.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase hrp conjugated secondary antibody, by Proteintech (1 mentions)
Crystal violet, by Solarbio (1 mentions)
Hoechst 33258, by Merck Group (1 mentions)
Rpmi 1640 medium, by Cytiva (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by CoWin Biotech (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Anti phospho akt ser473, by Cell Signaling Technology (1 mentions)
Bax 50599 2 lg, by Proteintech (1 mentions)
Gsh and oxidized glutathione disulfide gssg assay kit, by Beyotime (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by neoFroxx (1 mentions)
P53 10442 1 ap, by Proteintech (1 mentions)
Gapdh 10494 1 ap, by Proteintech (1 mentions)
Ecl detection system, by Bio-Rad (1 mentions)
Ab24551, by Abcam (1 mentions)
P0448, by Agilent Technologies (1 mentions)
P0447, by Agilent Technologies (1 mentions)
Ab6741, by Abcam (1 mentions)
Ab76026, by Abcam (1 mentions)
2 protocols using parp 13371 1 ap
1
Apoptosis Induction and Signaling Pathway
2
Quantitative Western Blot Analysis
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Cell lysates were prepared for Western blot analysis as previously described [54 (link)]. The proteins were probed with primary antibody against BQ323636.1 [29 (link)], NCOR2 (#ab24551, Abcam, Cambridge, UK), NRF2 (#NBP1-32822, Novus biologicals, Centennial, CO, USA), and phosphor-NRF2 (S40) (#ab76026, Abcam, Cambridge, UK). β-Actin (#sc-47778), Lamin B (#sc-6216) and NQO1 (#sc-32793) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against β-Tubulin (#86298S), DYKDDDDK Tag (#2368S) and HO-1 (#5853S) were purchased from Cell Signaling Technology (Danvers, MA, USA). PARP (#13371-1-AP) and Caspase 3 (#66470-2-Ig) antibodies were obtained from Proteintech group (Rosemont, IL, USA). Anti-His tag antibody was obtained from Wako (#010-21861, Osaka, Japan). Primary antibodies were detected using anti-mouse (#P0447, Dako, Glostrup, Denmark), anti-rabbit (#P0448, Dako, Glostrup, Denmark), or anti-goat HRP (#ab6741, Abcam, Cambridge, UK) conjugates as appropriate and visualised using the ECL detection system (Bio-Rad, Hercules, CA, USA). The original blots (Supplementary Figure S16 ) were scanned and densitometry readings/intensity ratio of each band calculated for analysis (Supplementary Figure S17 ).
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