Sb216763

Quercetin (purity ≥98.5%) and Temozolomide (TMZ) (purity ≥98%) were purchased from Aladdin (Shanghai, China) and dissolved in dimethyl sulfoxide (DMSO). Cell Counting Kit-8 was purchased from Bimake (Houston, TX, United States). Antibodies against GSK-3β, β-catenin, E-cadherin, N-cadherin, Vimentin, ZEB1, Akt, p-Akt and anti-rabbit IgG (Alexa Fluor^® 488 Conjugate) were purchased from Cell Signaling Technology (Danvers, MA, United States). Antibodies against β-actin, p-GSK-3β (Ser 9) were purchased from Santa Cruz (Dallas, Texas, United States). Horseradish peroxidase-conjugated goat anti-rabbit IgG, Horseradish peroxidase-conjugated goat anti-mouse IgG secondary antibodies and Phalloidin were purchased from Zhongshan Jinqiao Biotechnology Co. Ltd. (Beijing, China). Antibody against Ki67 was obtained from Affinity Biosciences (Zhenjiang, China). The SDS-PAGE Gel Quick Preparation Kit, RIPA lysis buffer, Crystal violet and BCA Protein Assay Kit were purchased from Beyotime Biotechnology (Shanghai, China). SB216763 was purchased from MedChemExpress (New Jersey, United States).

FuHeng Biology provides us with HCT116 cells for our research (Shanghai, China). China's Sino Biological provided the CXCR4-overexpressing vector (MG50621-UT). WAY-262611 and SB 216763 were acquired from MedChemExpress China, while Astragalus membranaceus (HQ) and Curcuma zedoaria (EZ) were obtained from Jiangsu Province Hospital of TCM, respectively. Sigma-Aldrich supplied the rest of the required agents.