CD11c+ DCs were isolated from the right iLNs of wild-type mice that had been treated with intradermal injections of PBS, LNP-R, or LNP-Col II-R on weeks 1 and 2. The isolated lymphocytes were stained with anti-CD11c MicroBeads UltraPure (Miltenyi Biotec, USA, 130-125-835) for 15 min and sorted by MACS bead column according to the manufacturer’s instruction (Miltenyi Biotec, USA). The sorted CD11c+ DCs (0.5 × 106 cells per animal) were transferred to OA mice through intradermal injection. Eight weeks after DC transfer, the joints were harvested for analysis.
Anti cd11c microbeads ultrapure
Manufactured by Miltenyi Biotec
Sourced in United States
Anti-CD11c MicroBeads UltraPure are magnetic beads coated with antibodies that specifically bind to the CD11c surface antigen. They are designed for the isolation and enrichment of CD11c-positive cells from various sample types.
Automatically generated - may contain errors
2 protocols using anti cd11c microbeads ultrapure
1
CD11c+ DC Transfer to Osteoarthritis Mice
2
Isolation and Modulation of Immune Cells
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Epithelial cells were isolated from colonic tissue using a dithiotheritol (DTT)/ethylenediaminetetraacetic (EDTA) buffer. Neutrophils were purified from mouse bone marrow using anti-Ly6G MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec). CD11c + cells were purified from mouse spleen using anti-CD11c MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec). Purity checks and cell counts were performed using a BD Accuri C6 flow cytometer (BD Biosciences). After purification, neutrophils were seeded in 96-well suspension plate (Sarstedt), rested for 30 min in RPMI 1640 Medium (Gibco, ThermoFisher Scientific) before addition of 2% heat-inactivated Fetal Bovine Serum (FBS), 2-DG 10 mM, oligomycine 1.5μM or H2O2 0.01M and incubated at 37°C as indicated. Isolation of LP immune cells was performed as previously described. 52
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