Delta 2 24 lsc

Manufactured by Martin Christ

Sourced in Germany

The Delta 2–24 LSC is a laboratory equipment product manufactured by Martin Christ. It is a freeze dryer designed for sample preparation and preservation. The core function of the Delta 2–24 LSC is to remove moisture from samples through a process of sublimation, which is the direct transformation of a solid to a gas without passing through a liquid phase.

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Lab products found in correlation

Dsc 7, by PerkinElmer (1 mentions) Ciprofloxacin hydrochloride, by MP Biomedicals (1 mentions)

4 protocols using delta 2 24 lsc

Mineral Clay-Based Composite Hydrogels

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Mineral clays were solubilized in ultrapure water and were kept under magnetically stirring for 15 h. Afterwards, the clay dispersion was ultrasonicated for 2 min. Type II collagen and the gentamicin solution were added and homogenized, at 24 °C, to obtain 100 g of gel for each sample in accordance with the composition given in Table 4.
The composite hydrogels were freeze-dried using Delta 2–24 LSC (Martin Christ, Osterode, Germany) instrument and spongious forms were obtained and characterized. The obtained 3D composite biomaterials can be observed in Figure 12.
The freeze-dried composite scaffolds were evaluated by Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, differential scanning calorimetry, thermo gravimetric analysis, swelling ratio, biodegradation ratio, mechanical tests, drug release, antimicrobial tests, and cellular viability.

Marin M.M., Ianchis R., Leu Alexa R., Gifu I.C., Kaya M.G., Savu D.I., Popescu R.C., Alexandrescu E., Ninciuleanu C.M., Preda S., Ignat M., Constantinescu R, & Iovu H. (2021). Development of New Collagen/Clay Composite Biomaterials. International Journal of Molecular Sciences, 23(1), 401.

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Thermal Analysis of Dried Fecal Samples

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The same fecal samples (n = 3 per treatment), as previously described in digestive enzyme section, were dried using a freeze dryer (Delta 2-24 LSC; Martin Christ Gefriertrocknungsanlagen GmbH, Osterode am Harz, Germany) for 24 h. The dried samples were mashed to obtain homogenous powder using mortar and pestle. The thermal response of three milligrams dried feces was run from 40 to 400 °C at a rate of 10 °C min⁻¹ against an empty reference pan. Thermograms and thermal properties, in terms of onset (T_o), peak (T_p), and conclusion (T_c) temperatures and enthalpy (∆H), were recorded using a differential scanning calorimeter (DSC7; Perkin Elmer, Waltham, MA, USA).

Jualaong S., Kanghae H., Thongprajukaew K., Saekhow S., Amartiratana N, & Sotong P. (2021). Optimal Feeding Frequency for Captive Hawksbill Sea Turtle (Eretmochelys imbricata). Animals : an Open Access Journal from MDPI, 11(5), 1252.

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Collagen Sponges with Ciprofloxacin

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Collagen-based sponges were prepared starting from a collagen gel of 1.91% and acidic pH. This gel was obtained from bovine derma using a current technology at INCDTP—Division Leather and Footwear Research Institute, Collagen Department (Bucharest, Romania) [40 (link),41 ]. Ciprofloxacin Hydrochloride (MP Biomedicals, LCC, Solon, OH, USA) was added in concentration of 0.5; 0.75 and 1 g/g to a collagen solution (1%, pH 7.4) prepared from the initial gel with 1 M sodium hydroxide. The obtained solutions were further cross-linked with 0.5% glutaraldehyde reported to dry collagen. The sponge materials were obtained by lyophilization process consisting of three steps: (i) freezing at −55 °C for 1 min; (ii) drying at −55 °C for 15 h—time in which approximately 90% of water is sublimated; and (iii) final-drying at −40 °C for 10 min. The sublimation process took place under vacuum and was performed using Delta 2-24 LSC (Martin Christ, Osterode am Harz, Germany) equipment. All obtained collagen sponges of 2 cm diameter and 0.5 cm thickness were packed in polyethylene bags and sterilized at 254 nm using Vilber–Lourmat equipment (Suebia, Germany).

Ioan D.C., Rău I., Albu Kaya M.G., Radu N., Bostan M., Zgârian R.G., Tihan G.T., Dinu-Pîrvu C.E., Lupuliasa A, & Ghica M.V. (2020). Ciprofloxacin-Collagen-Based Materials with Potential Oral Surgical Applications. Polymers, 12(9), 1915.

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Preparation of Collagen-Based Hydrogels

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Acidic collagen hydrogels (pH of about 4) with and without TA and CHDG were prepared as described elsewhere [56 ]. Briefly, a series of nine collagen hydrogels (pH 4) of 1.10% collagen and different contents of TA and CHDG (by taking into account all combinations of 5, 10, 15% TA and 1.82, 4.55, 9.09% CHDG, the concentrations being considered with respect to the collagen) were prepared by adding proper amounts of aqueous solutions of TA and/or CHDG to a certain quantity of initial collagen hydrogel (2.50% and pH 3.20) under gentle stirring. To maintain the overall pH of the systems at ca. 4 during the collagen-based hydrogels preparation, a solution of 1M NaOH was used. A series of three collagen hydrogels containing TA (5, 10, and 15%) for comparison were also prepared. All the collagen-based hydrogels were equilibrated by keeping them at 4 °C for 24 h. The final porous collagen-based matrices were obtained by freeze-drying collagen-based hydrogels (poured into molds with a flat bottom and open to the air side on top) for 48 h, employing a Delta 2–24 LSC (Martin Christ, Osterode am Harz, Germany) freeze-dryer. A detailed algorithm of the entire lyophilization process was described previously [57 ].

Brăzdaru L., Staicu T., Albu Kaya M.G., Chelaru C., Ghica C., Cîrcu V., Leca M., Ghica M.V, & Micutz M. (2022). 3D Porous Collagen Matrices—A Reservoir for In Vitro Simultaneous Release of Tannic Acid and Chlorhexidine. Pharmaceutics, 15(1), 76.

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