Pe cy7 anti cd127

Anti-CD14 conjugated to violet blue was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). FITC-anti-CD4, PE Cy5-anti-CD11c, PE-anti-CD25, BV650-anti-CD25, APC-anti-CD45RA, BV711-anti-CD15s, PE-anti-CD45RA, PC5-anti-CD28, and PE anti-CTLA-4 were obtained from BD Biosciences (Mountain View, CA, USA). PE CY7 anti-CD127, BV421-anti CCR7, PE-anti-CD59, and BV510-anti-CD27 were purchased from Sony Biotechnology (San Jose, CA, USA); PE-FOXP3, APC-FOXP3, and eF450-Helios were supplied by eBiosciences (San Diego, CA, USA); PC5 anti-ICOS, APC-anti-PD1, BV421-anti-CD39, APC-anti-GITR, and BV421-anti LAG3 were purchased from Biolegend (San Diego, CA, USA); and staphylococcal enterotoxin E (SEE) was purchased from Toxin Technology Inc.; and CellTrace carboxyfluorescein succinimidyl ester (CFSE) was purchased from Molecular Probes (Eugene, OR, USA). The anti-CD3 (OKT3) used for some proliferation assays was purchased from Biolegend (San Diego, CA, USA).

For the sorting experiments, FITC-coupled anti-CD4 and PE-coupled anti-CD25 were purchased from BD Biosciences (MountainView, CA, USA), APC anti-CD45RA was purchased from BioLegend (San Diego, CA, USA), PE-Cy5 anti-CD11c was purchased from Beckman Coulter (Fullerton, CA, USA), BV650 anti-CD3, PE-Cy7 anti-CD127 and BV510 anti-CD16 were purchased from Sony Biotechnology (San Jose, CA, USA), and Violet Blue anti-CD14 was purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). For the proliferation assays, SEE was purchased from Toxin Technology Inc (Sarasota, FL, USA), CellTrace^™ carboxyfluorescein succinimidyl ester (CFSE) was purchased from Molecular Probes (Eugene, OR, USA) and CellTrace^™ Violet (CTV) was purchased from Invitrogen (Carlsbad, CA, USA). For phenotyping, PE-anti-PD-L1, PE-anti-BTLA, PE-anti-LAG3, PE-anti-TIM3, APC-anti-PD-1, and APC-anti-4-1BB were purchased from BioLegend (San Diego, CA, USA), APC anti-ICOS was purchased from Invitrogen (Carlsbad, CA, USA) and APC anti-TIGIT was purchased from Sony Biotechnology (San Jose, CA, USA). PE-anti-PD-L2 and anti-CTLA-4 were purchased from ebiosciences (San Diego, CA, USA).