Plvx mcmv zsgreen ires puro vector

Manufactured by Addgene

Sourced in United States

The PLVX-mCMV-ZsGreen-IRES-Puro vector is a lentiviral expression vector that allows for stable expression of a gene of interest and a puromycin resistance gene. The vector contains the mCMV promoter to drive expression of the ZsGreen fluorescent protein and the IRES sequence to enable co-expression of the puromycin resistance gene.

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Lab products found in correlation

Plvx shrna2 puro vector, by Addgene (2 mentions) Pmirglo dual luciferase reporter vector, by Promega (1 mentions) Pcdna3, by Promega (1 mentions) Mir 193b 3p inhibitor, by RiboBio (1 mentions) Pcdna3.1 vector, by Promega (1 mentions)

Spelling variants of this product

PLVX-puro (16 citations) PLVX-Puro vector (9 citations) PLVX-IRES-Puro vector (5 citations) PLVX-IRES-Puro (4 citations) Plvx-IRES-zsgreen (2 citations)

2 protocols using plvx mcmv zsgreen ires puro vector

PPARGC1A Overexpression and Targeting

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For PPARGC1A overexpression vector construction, the coding sequence of PPARGC1A was amplified and cloned into pcDNA-3.1 (Promega, Madison, WI, USA) between the HindIII and XhoI sites. Specific siRNA against PPARGC1A and non-specific siRNA negative control (NC) were designed and synthesized by Guangzhou RiboBio (Guangzhou, China).
For overexpression lentiviral vectors constructed, PPARGC1A coding sequence was amplified and then cloned into the pLVX-mCMV-ZsGreen-IRES-Puro vector (Addgene, Cambridge, MA, USA) between the SpeI and NotI sites. Short hairpin RNAs (shRNAs) against PPARGC1A were designed and then subcloned into the pLVX-shRNA2-Puro vector (Addgene, Cambridge, MA, USA) between the BamHI and EcoRI sites.
For pmirGLO dual-luciferase miRNA target reporter vectors constructed, the segment sequence of the PPARGC1A 3′ UTR that contained the putative miR-193b-3p binding sequence was amplified and then cloned into the pmirGLO dual-luciferase reporter vector (Promega, Madison, WI, USA) between the NheI and XhoI sites. Mutant plasmids were generated by changing the binding site of miR-193b-3p from GGCCAGT to TTAAGAC.
MiR-193b-3p mimic, mimic NC, miR-193b-3p inhibitor, and inhibitor NC were designed and synthesized by Guangzhou RiboBio (Guangzhou, China).
The primers and oligonucleotide sequences used in this study are listed in Tables S1 and S2.

Ma M., Cai B., Kong S., Zhou Z., Zhang J., Zhang X, & Nie Q. (2022). PPARGC1A Is a Moderator of Skeletal Muscle Development Regulated by miR-193b-3p. International Journal of Molecular Sciences, 23(17), 9575.

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Molecular Constructs for LncEDCH1 and SERCA2

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For FLAG fusion protein construction, six ORFs of LncEDCH1 were amplified and cloned into the pcDNA3.1-3xFLAG-C vector. For LncEDCH1 and SERCA2 overexpression plasmid construction, the full-length sequence LncEDCH1 and the SERCA2 coding sequence were amplified by PCR and cloned into a pcDNA3.1 vector (Promega).
For construction of viral vectors, the full-length sequence of LncEDCH1 was amplified and then cloned into a pLVX-mCMV-ZsGreen-IRES-Puro vector (Addgene). Short hairpin RNA (shRNA) against LncEDCH1 was designed by Shanghai Hanbio Biotechnology and subcloned into the pLVX-shRNA2-Puro vector (Addgene).
LncEDCH1 is an lncRNA molecule mainly present in the cytoplasm; the siRNAs that were used for the specific knockdown of LncEDCH1 and SERCA2 (NCBI: NM_001271973.1) were designed and synthesized by Guangzhou RiboBio.

Cai B., Ma M., Zhang J., Wang Z., Kong S., Zhou Z., Lian L., Zhang J., Li J., Wang Y., Li H., Zhang X, & Nie Q. (2021). LncEDCH1 improves mitochondrial function to reduce muscle atrophy by interacting with SERCA2. Molecular Therapy. Nucleic Acids, 27, 319-334.

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