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Anti nk 1.1 mab

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Anti-NK 1.1 mAb is a monoclonal antibody that binds to the NK 1.1 antigen expressed on natural killer cells in mice. It is used as a tool for the identification and study of natural killer cells in laboratory research.

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Lab products found in correlation

Poly 1 c, by InvivoGen (1 mentions) Anti asialo gm1, by Fujifilm (1 mentions) Easysep mouse nk cell enrichment kit, by STEMCELL (1 mentions) Il 15, by Thermo Fisher Scientific (1 mentions)

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Anti-NK (2 citations)

2 protocols using anti nk 1.1 mab

1

Poly(I:C)-induced Inflammation in B6 Mice

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Two forms of B6 mice, C57BL/6 wild-type B6 mice (females) and CR3−/− and TLR3−/− B6 mice, were treated intraperitoneally with 200 μg of poly(I:C) bought from InvivoGen (San Diego, CA). poly(I:C) was dissolved in 200 μl PBS prior to injection. One injection of 50 μl of anti-asialo GM1 (AGM1) polyclonal antibody (Sigma-Aldrich, San Diego, CA) was given to the wild-type B6 mice to deplete them of NK cells. This was injected between 6 hr and 12 hr prior to their injection with poly(I:C). Flow cytometry was employed to confirm NK cell depletion by staining with anti-NK 1.1 mAb (Pharmingen, San Diego, CA, USA).
Salem M.L., El-Naggar S.A., Mobasher M.A, & Elgharabawy R.M. (2020). The Toll-Like Receptor 3 Agonist Polyriboinosinic Polyribocytidylic Acid Increases the Numbers of NK Cells with Distinct Phenotype in the Liver of B6 Mice. Journal of Immunology Research, 2020, 2489407.
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2

Depletion of NK cells and HSV1 infection

Cited 1 time
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Depletion of NK cells 1 day before HSV1 infection were performed by anti-asialo GM1 (Wako Pure Chemicals, Richmond, VA) or normal rabbit IgG (Cell Signaling Techonology, Danvers, MA), or anti-NK1.1 mAb (BD Pharmingen) or control rat IgG2a (BD Pharmingen), as described previously14 (link). Skin tissues were harvested on days 0 (before infection), 2, 4 and 7 postinfection. Splenocytes from NC/Nga mice were negatively selected by EasySep Mouse NK cell Enrichment Kit (STEMCELL Technologies). These NK-enriched cells were cultured in IL-15 (PeproTech) for 4 days. The cultured NK cells (purity ≥93%) were checked by flow cytometry after staining with anti-NK1.1 and anti-CD3 (BD Pharmingen) antibodies and intravenously transferred (8–10×106 per mouse).
Kawakami Y., Ando T., Lee J.R., Kim G., Kawakami Y., Nakasaki T., Nakasaki M., Matsumoto K., Choi Y.S, & Kawakami T. (2016). Defective NK cell activity in a mouse model of eczema herpeticum. The Journal of allergy and clinical immunology, 139(3), 997-1006.e10.
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