LPS (Escherichia coli 055: B5), methoxyamine hydrochloride, fatty acid methyl esters (C7-C30, FAMEs), anhydrous pyridine, and anhydrous sodium sulfate were purchased from Sigma-Aldrich (St. Louis, MO, USA). PBS, the derivatization reagent N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA; containing 1% chlorotrimethylsilane (TMCS)), methanol (Optima; LC-MS grade), and n-ethane were purchased from Thermo Fisher (Fair Lawn, NJ, USA). Dichloromethane, chloroform, and acetone were purchased from the China National Pharmaceutical Group Corporation. An AXSYM automatic immunoassay analyser for liver and kidney function tests was purchased from Abbott Laboratories, Illinois, USA. Ultrapure water was obtained from a Millipore Reference ultrapure water system (Billerica, MA, USA) equipped with a liquid chromatography-coupled 0.22 μm filter. A GC-TOFMS system was purchased from LECO Corp. (St. Joseph, MI, USA).
Reference ultrapure water system
Manufactured by Merck Group
Sourced in United States
The Reference ultrapure water system is a laboratory equipment designed to produce highly purified water for various applications. It utilizes a multi-stage filtration process to remove impurities, resulting in water of exceptional purity and quality.
Automatically generated - may contain errors
Lab products found in correlation
Anhydrous sodium sulfate, by Merck Group (4 mentions)
Methoxyamine hydrochloride, by Merck Group (2 mentions)
N ethane, by Thermo Fisher Scientific (2 mentions)
Anhydrous pyridine, by Merck Group (2 mentions)
Gc tofms, by Leco (2 mentions)
N hexane, by Thermo Fisher Scientific (2 mentions)
High purity methoxyamine hydrochloride, by Merck Group (2 mentions)
Mstfa containing 1 tmcs, by Thermo Fisher Scientific (2 mentions)
Methanol optima lc ms, by Thermo Fisher Scientific (2 mentions)
Gc tof ms system, by Leco (1 mentions)
Ethylene glycol, by Thermo Fisher Scientific (1 mentions)
Urea, by Thermo Fisher Scientific (1 mentions)
Choline chloride, by Thermo Fisher Scientific (1 mentions)
Hydrochloric acid (hcl), by Avantor (1 mentions)
Nitric acid hno3, by Chem-Lab (1 mentions)
Chloral hydrate powder, by Sinopharm (1 mentions)
Paraformaldehyde (pfa), by Sinopharm (1 mentions)
5 protocols using reference ultrapure water system
1
Metabolic Profiling of LPS-Induced Inflammation
2
Reagents and Materials for Analytical Methods
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Choline chloride (99%), ethylene glycol (99.5%), urea (99.5%) and Triton X-10 were purchased from Acros Organics NV (Geel, Belgium). Levulinic acid (99%) was purchased from J&K Scientific BVBA (Pforzheim, Germany). Tetrabutylammonim chloride (97 wt%) was purchased from Sigma Aldrich (Steinheim, Germany). Tetrabutylphosphonium chloride (>95 wt%) was obtained from Io-Li-Tec (Heilbronn, Germany). Nitric acid, HNO3, (>65%) and anhydrous calcium chloride, CaCl2, (>95%) were purchased from Chem-Lab NV (Zedelgem, Belgium). Hydrochloric acid, HCl, (>37%) was purchased from VWR (Fontenay-sous-Bois, France). A silicon solution in isopropanol was purchased from SERVA Electrophoresis GmbH (Heidelberg, Germany). The standard solutions (1000 μg mL−1) of zinc, iron, copper, lead, calcium, arsenic, lutetium, niobium and platinum were purchased from Chem-Lab (Zedelgem, Belgium). Water was always of ultrapure quality, deionized to a resistivity of 18.2 MΩ cm with a Millipore Reference+ ultrapure water system. All chemicals were used as received without any further purification. The goethite residue was kindly provided by Nyrstar (Balen, Belgium).
3
Untargeted Metabolomic Analysis by GC-TOF/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
High-purity methoxyamine hydrochloride, fatty acid methyl ester (C7–C30, FAME), anhydrous pyridine (99.5%), and anhydrous sodium sulfate were obtained from Sigma–Aldrich (MO, USA). Derivatization reagents MSTFA (containing 1% TMCS), methanol (Optima LC-MS), and n-hexane were purchased from Thermo Fisher (NJ, USA). Dichloromethane (99.5%), chloroform (99%), and acetone (99.5%) were purchased from China National Pharmaceutical Group Corporation (Beijing, China). Ultrapure water was prepared from a Millipore Reference ultrapure water system (MA, USA) equipped with an LC-MS filter. GC-TOF/MS (LECO Corp, MI, USA) based on silanization-derived GC-TOF/MS was used as an analytical platform for untargeted metabolomics.
4
GC-TOF/MS-based Untargeted Metabolomics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
High-purity methoxyamine hydrochloride, fatty acid methyl ester (C7–C30, FAME), anhydrous pyridine (99.5%), and anhydrous sodium sulfate were obtained from Sigma-Aldrich (MO, USA). Derivatization reagents MSTFA (containing 1% TMCS), methanol (Optima LC-MS), and n-hexane were purchased from Thermo Fisher (NJ, USA). Dichloromethane (99.5%), chloroform (99%), and acetone (99.5%) were purchased from China National Pharmaceutical Group Corporation (Beijing, China). Ultrapure water was prepared from a Millipore Reference ultrapure water system (MA, USA) equipped with an LC-MS filter. GC-TOF/MS (LECO Corp, MI, USA) based on silanization-derived GC-TOF/MS was used as an analytical platform for untargeted metabolomics.
5
Metabolic Profiling of Bacterial Endotoxin Response
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LPS (Escherichia coli 055: B5), methoxyamine hydrochloride, fatty acid methyl ester (C7-C30, FAMEs), anhydrous pyridine, and anhydrous sodium sulfate were purchased from Sigma-Aldrich (St. Louis, MO, USA). PBS, derivatization reagent MSTFA (containing 1% TMCS), methanol (Optima LC-MS), and n-ethane were purchased from Thermo Fisher (Fair Lawn, NJ, USA). Dichloromethane, chloroform and acetone were purchased from the China National Pharmaceutical Group Corporation, Peking, China. Chloral hydrate powder and paraformaldehyde were purchased from Sinopharm Chemical Reagent Co., Ltd, Shanghai, China. The AXSYM automatic immunoassay analyzer for liver and kidney function tests was purchased from Abbott Laboratories, Illinois, USA. Experimental ultrapure water was prepared from a Millipore Reference ultrapure water system (Billerica, MA, USA) equipped with a liquid chromatography coupled 0.22 μm filter. A GC-TOFMS spectrometer was purchased from (LECO Corp., St. Joseph, MI, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!