Primary antibodies used were from Cell Signalling unless otherwise mentioned: β-actin (Abcam #ab6276), BrdU (BD Pharmingen #555627), cleaved caspase 3 (#9664), CDK1 (Abcam #ab18), CDK1 Y15 (#9111), CHK1 (#2360), CHK1 S296 (#2349), CHK1 S345 (#2348), ENT1 (Abcam #ab135756), H2AX (#7631), H2AX S139 (Millipore #05-636), H3 (#9715) and H3 S10 (#3377). For secondary antibodies, Alexa 488 (#4408, #4412) and Alexa 647 (#4410, #4414) from Cell Signalling were used in immunostaining. IRDye800-conjugated (#925-32210, #926-33210) and IR680-conjugated (#926-68070, #926-68021) antibodies from LICOR were used in immunoblotting.
Cdk1 y15
Manufactured by Abcam
Sourced in United States
CDK1 Y15 is a laboratory product that targets the Cyclin-dependent kinase 1 (CDK1) protein. CDK1 is a key regulator of cell cycle progression and its activity is modulated by phosphorylation at specific residues, including tyrosine 15. This product can be used in research applications to study cell cycle regulation and related cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Alexa 647, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Abcam (1 mentions)
Bromodeoxyuridine (brdu), by BD (1 mentions)
β actin, by Abcam (1 mentions)
Alexa 488, by Cell Signaling Technology (1 mentions)
Bcl 2, by Abcam (1 mentions)
Gapdh, by Abcam (1 mentions)
Bcl2 associated x (bax), by Abcam (1 mentions)
Lc3 2, by Abcam (1 mentions)
C parp, by Abcam (1 mentions)
Lc3 1, by Abcam (1 mentions)
Ecl kit, by Thermo Fisher Scientific (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Chemidoc system, by Bio-Rad (1 mentions)
Cdc25c, by Abcam (1 mentions)
Ripa lysis solution, by Thermo Fisher Scientific (1 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Cyclinb, by Abcam (1 mentions)
2 protocols using cdk1 y15
1
Molecular Markers for Cell Cycle and Apoptosis
2
Protein Expression Analysis in Lung Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In order to create protein lysates from MRC-5, A549, Calu-3, H1975, and H2228, protease and phosphatase inhibitors were added to radioimmunoprecipitation assay (RIPA) lysis solution (Thermo Fisher Scientific). The bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific) was used to measure the amounts of proteins. After sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for electrophoresis, polyvinylidene fluoride (PVDF) membranes (Millipore, Burlington, MA, USA) were transferred. Immunoblotting was carried out using primary antibodies against CDC25C, Bax, Bcl-2, LC3 I, LC3 II, p62, cyclin B, CDK1-Y15, CDK1, c-PARP, and GAPDH (all at 1:1,000; Abcam, Cambridge, MA, USA). Post-washing thrice with tris-buffered saline with Tween 20 (TBST), membranes were processed for band visualization using an enhanced chemiluminescence (ECL) kit (Thermo Fisher Scientific). Using the ChemiDoc system from Bio-Rad (Hercules, CA, USA), the intensities of the protein bands were recorded and subsequently analyzed with ImageJ software (National Institutes of Health, Bethesda, MD, USA).
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