E2 and Te were from Sigma-Aldrich (Saint-Louis, MO, USA). Estriol, estrone, and deuterated internal standards, 17-β estradiol (E2-D5) and testosterone (Te-D3) were from Cerilliant (Round Rock, TX, USA). All compounds had a purity greater than 99%. 17-OH-progesterone, aldosterone, androstenedione, corticosterone, cortisol, cortisone, 11-deoxycortisol, dehydroepiandrosterone, dehydroepiandrosterone sulfate, dihydrotestosterone, and progesterone were from Chromsystems Steroid Panel I & II kits (Grafelfing, Germany). Water, methanol, acetonitrile, 2-propanol, acetone, and acetic acid (LCMS grade) were from Fisher Scientific (Fair Lawn, NJ, USA). Calibrators and quality controls were from Chromsystems Steroid Panel II kit previously mentioned. Ultra-low steroid human serum (DC Mass Spect Gold) was from Golden West Biologicals, Inc. (Temecula, CA, USA) and is certified to contain < 0.03 pg/mL and < 0.025 ng/dL E2 and Te, respectively. Serum separator and clot activator blood collection tubes were obtained from Beckton Dickenson (Franklin Lakes, NJ, USA).
Progesterone
Manufactured by Chromsystems
Sourced in Germany
Progesterone is a laboratory equipment designed for the measurement of progesterone levels. It is a chemical compound used in various analytical and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Corticosterone, by Chromsystems (2 mentions)
11 deoxycortisol, by Chromsystems (2 mentions)
Acetone, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
2 propanol, by Thermo Fisher Scientific (1 mentions)
Acetic acid lc ms grade, by Thermo Fisher Scientific (1 mentions)
Progesterone d9, by Merck Group (1 mentions)
13c3 androstenedione, by Merck Group (1 mentions)
Cortisol d4, by Merck Group (1 mentions)
2 protocols using progesterone
1
Reliable Steroid Profiling via Mass Spectrometry
2
Quantifying Steroids in Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ethyl ether and HPLC-grade water were supplied by CARLO ERBA (Italy). HPLC-grade methanol (MeOH) was purchased from Thermo Fischer Scientific.
6 PLUS1 Multilevel Serum Calibrator Set MassChrom Steroids Panel 1&2 allowing the simultaneous quantification of 7 steroids (progesterone, 11-deoxycorticosterone, corticosterone, 17OHP, 11-deoxycortisol, cortisol and androstenedione) among the proposed panel of 15 steroids was purchased from Chromsystems (Germany), as well as internal quality controls (QC) MassCheck Steroid Panel 1&2 Serum Control.
Internal standards: corticosterone-D8 and 11-deoxycorticosterone-D8 were purchased from LGC (Teddington, UK) and separately dissolved in MeOH to get stock solutions at 1 mg/mL, stored at −20°C. Internal standard solutions of cortisol-D4, 11-deoxycortisol-D5, androstenedione-13C3, 17OHP-D8 and progesterone-D9 were purchased from Sigma-Aldrich. A mix of the 7 internal standard solutions was further prepared in MeOH.
6 PLUS1 Multilevel Serum Calibrator Set MassChrom Steroids Panel 1&2 allowing the simultaneous quantification of 7 steroids (progesterone, 11-deoxycorticosterone, corticosterone, 17OHP, 11-deoxycortisol, cortisol and androstenedione) among the proposed panel of 15 steroids was purchased from Chromsystems (Germany), as well as internal quality controls (QC) MassCheck Steroid Panel 1&2 Serum Control.
Internal standards: corticosterone-D8 and 11-deoxycorticosterone-D8 were purchased from LGC (Teddington, UK) and separately dissolved in MeOH to get stock solutions at 1 mg/mL, stored at −20°C. Internal standard solutions of cortisol-D4, 11-deoxycortisol-D5, androstenedione-13C3, 17OHP-D8 and progesterone-D9 were purchased from Sigma-Aldrich. A mix of the 7 internal standard solutions was further prepared in MeOH.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!