H&E staining was performed as described by Grosset et al., 2017 [50 ]. Briefly, hematoxylin (Fisher Scientific, Canada) was added on cells, and a counterstaining for 30 seconds was performed followed by a water rinse for 5 minutes. Slides were then dipped in 50% (vol/vol) alcoholic eosin Y solution (Leica Microsystems, Canada) then rinsed in ethanol before slide mounting.
Alcoholic eosin y solution
Manufactured by Leica
Sourced in Canada
Alcoholic eosin Y solution is a laboratory reagent used as a staining agent in various histological and cytological procedures. It is a pink-colored dye solution made by dissolving eosin Y, a fluorescent dye, in an alcoholic solvent. The core function of this solution is to provide a contrasting stain that highlights specific cellular and tissue structures for microscopic examination and analysis.
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Lab products found in correlation
2 protocols using alcoholic eosin y solution
1
Hematoxylin and Eosin Staining
2
Staining Infected Macrophages with H&E
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Activated macrophages seeded onto coverslips in six well-plates were subjected to H&E staining. Following infection with L. tropica promastigotes at the ratio of one parasite/3 macrophages, and treatment for 24 h with either HAS1-F1 or HAS1-F2, cells were fixed with methanol for 1 min. The coverslips were then covered in Hematoxylin (Fisher Scientific, Canada) and a counterstaining was performed for 30 s before rinsing for 5 min with water. Subsequently, the coverslips were dipped in an alcoholic eosin Y solution (Leica Microsystems, Canada), and rinsed with deionized water before mounting on slides.
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