Plan apo objective na 1

Manufactured by Hamamatsu Photonics

The 100x Plan-Apo objective NA 1.4 is a high-numerical aperture microscope objective designed for Hamamatsu Photonics' laboratory equipment. It features a magnification of 100x and a numerical aperture of 1.4, which allows for high-resolution imaging and a large depth of field.

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Lab products found in correlation

Flash 4, by Hamamatsu Photonics (1 mentions) Spectra x, by Hamamatsu Photonics (1 mentions) Axiophot microscope, by Zeiss (1 mentions) Orca er camera, by Hamamatsu Photonics (1 mentions) Axiovert 200, by Zeiss (1 mentions)

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Plan-Apo (7 citations) Plan-Apo objective (4 citations) 60x/1.4NA Plan Apo (2 citations)

2 protocols using plan apo objective na 1

Microscopic Imaging of Cellular Dynamics

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Cells were transferred onto Concanavalin A-treated MatTek dishes (MatTek Corp., Ashland, MA) and visualized at room temperature Microscopy was performed using an inverted epi-fluorescence microscope (Nikon Ti) equipped with a Spectra X LED light source and a Hamamatsu Flash 4.0 sCMOS camera using a 100x Plan-Apo objective NA 1.4 and the NIS Elements software. Representative images were processed using ImageJ software. Brightness and contrast were adjusted to the same values for images belonging to the same experiment and were chosen to cover the whole range of signal intensities. Image processing for PB analysis was performed using Diatrack 3.05 particle tracking software (Vallotton and Olivier, 2013 (link)) as described below.

Sachdev R., Hondele M., Linsenmeier M., Vallotton P., Mugler C.F., Arosio P, & Weis K. (2019). Pat1 promotes processing body assembly by enhancing the phase separation of the DEAD-box ATPase Dhh1 and RNA. eLife, 8, e41415.

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Fluorescence Microscopy Protocol for Cell Imaging

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An Axiovert 200 or an Axiophot Zeiss microscope was used, equipped with a 100X PlanApo objective (NA 1.4) and Chroma 83000 multi-bandpass dichroic and emission filter sets (Brattleboro, VT), set up in a wheel to prevent optical shift, with an Orca-ER camera (Hamamatsu, NJ) or a cooled charge-coupled device (CCD) camera (200 series, Photometrics). Where required, a narrow band-pass fluorescein filter was inserted to correct for any bleed-thru of Texas-red fluorescence into the fluorescein channel. Most experiments were carried out a minimum of 3 times, with typically 50–100 cells scored in each experiment. Key results were confirmed by at least two independent investigators. Images were minimally enhanced for brightness and contrast to resemble what was seen by eye through the microscope (unless otherwise noted in the text/legends). Digital imaging software (Volocity from Perkin Elmer & Metamorph) was used to quantify signals (see Supplement for details).

Hall L.L., Carone D.M., Gomez A., Kolpa H.J., Byron M., Mehta N., Fackelmayer F.O, & Lawrence J.B. (2014). Stable CoT-1 repeat RNA is abundant and associated with euchromatic interphase chromosomes. Cell, 156(5), 907-919.

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