Rbl 2h3

The rat basophilic leukemia cell line RBL-2H3 was supplied from the Korean Cell Line Bank (Seoul, South Korea). RBL-2H3 cells were cultured in Minimum Essential Medium (MEM) (Welgene, South Korea) supplemented with 10% FBS (Gibco, Rockville, MD, United States) and 1% penicillin-streptomycin under an atmosphere of 5% CO₂ in a humidified 37°C incubator. RAW264.7 macrophage cells, IgEL b4 cells and 293 T cells were purchased from American Type Culture Collection (Manassas, VA, United States) and were cultured in Dulbecco Modified Eagle’s medium (DMEM) with 10% FBS and 1% penicillin-streptomycin. Calcium ionophore A23187 and phorbol 12-myristate 13-acetate (PMA) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Quercetin, 1-Chloro-2,4-dinitrobenzene (DNCB) and dexamethasone were purchased from Sigma-Aldrich (St. Louis, MO, United States). Albumin from bovine serum, 2,4-dinitrophenylated (DNP-BSA) was purchased from Invitrogen by Thermo Fisher Scientific (Eugene, OR, United States). rIL-4 and mTNF-α were purchased from R&D system Inc (Minneapolis, MN, United States). mIL-6 was purchased from Biolegend (San Diego, CA, United States).
Stock solutions of ZCO were diluted using the mixture solution of DMSO and ethanol (1:1) to a concentration of 20% and filter-sterilized, and then diluted with phosphate buffered saline (PBS) for the working concentration.

Raw264.7 as mouse macrophage cell and RBL-2H3 as rat mast cell were obtained from the Korean Cell Line Bank (Seoul, Korea). Raw264.7 and RBL-2H3 cells were maintained in DMEM contained supplemented with 10% FBS and 1% P/S at 37 °C in a 5% carbon dioxide (CO₂) incubator. Culture medium was changed every two days, and cell density was maintained at 80–90% during subculture.

Murine, colon 26 (CT26), human, SW480 and SW620, CRC cells, rat mast cells (RBL-2H3), and mouse macrophage (RAW264.7) cell lines were obtained from the Korean Cell Line Bank (Seoul, South Korea). CT26, RBL-2H3, and RAW264.7 cells were cultured in Dulbecco’s modified Eagle’s medium. SW480 and SW620 cells were cultured in RPMI 1640 medium. All culture media (Gibco BRL, Grand Island, NY, USA) were supplemented with 10% fetal bovine serum (Gibco BRL, Grand Island, NY, USA), 1% penicillin, and streptomycin. All cells were maintained at 37 °C in a humidified incubator containing 5% CO₂.

Dexamethasone (Dexa), anti-dinitrophenyl (DNP) IgE, DNP-human serum albumin (HSA), ovalbumin (OVA), and Histodenz were purchased from Sigma-Aldrich (St. Louis, MO), and alum adjuvant was purchased from Thermo Fisher Scientific (Waltham, MA). Rat basophilic leukemia (RBL-2H3) cells purchased from Korean Cell Line Bank (Seoul, Republic of Korea). RBL-2H3, rat peritoneal mast cells (RPMC), and mouse bone marrow-derived mast cells (mBMMC) were grown at 37°C in 5% CO₂ in Dulbecco's modified Eagle's medium, α-minimum essential medium (Gibco, Grand Island, NY) and RPMI 1640 (Hyclone, Logan, UT), respectively, supplemented with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin G, 100 μg/mL streptomycin, and 250 ng/mL amphotericin B. In addition, complete RPMI 1640 media was supplemented by 4 mM L-glutamine, 25 M HEPES, 50 μM 2-mercaptoethanol, 1 mM sodium pyruvate, MEM non-essential amino acid solution (Gibco), 10 ng/mL murine IL-3, and 2 ng/mL murine SCF (PeproTech, Rocky Hill, NJ). RBL-2H3 were used throughout the study at passages ranging from 4 to 8.