Glow-discharged EM grids coated with Formvar/Carbon (EMS) were floated on a 20-μl drop of purified exosome fraction. The excess fluid was blotted away using filter paper, and the exosomes adhering to the grid surface were immune-labeled with mouse monoclonal anti-CD81 antibody (Santa Cruz Sc-7637) followed by goat anti-mouse secondary antibody coupled with 6 nm gold (EMS). Finally, the grids were fixed with 1% glutaraldehyde (EMS), washed, and embedded in a thin film of uranyl acetate-methylcellulose (a 4% uranyl acetate and 2% methylcellulose mixture in a 1:9 ratio) using the wire loop technique. Samples were analyzed under a JEOL transmission electron microscope (JEM-1010) operating at 80 kV and equipped with an SIA model 12C 4K CCD camera.
Mouse monoclonal anti cd81 antibody
Manufactured by Santa Cruz Biotechnology
The Mouse monoclonal anti-CD81 antibody is a laboratory reagent used to detect the presence of the CD81 protein. CD81 is a cell surface protein that belongs to the tetraspanin family and is expressed on various cell types. This antibody can be used in techniques such as flow cytometry, immunohistochemistry, and Western blotting to identify and study cells expressing CD81.
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Lab products found in correlation
2 protocols using mouse monoclonal anti cd81 antibody
1
Exosome Immunolabeling and Visualization
2
Immunogold Labeling of Exosomes
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Glow-discharged EM grids coated with formvar-carbon (EMS) were oated on a 20-µL drop of puri ed exosome fraction. Excess uid was blotted away by lter paper and exosomes adhering to the grid surface were immune-labeled with mouse monoclonal anti-CD81 antibody (Santa Cruz Sc-7637) followed by goat anti-mouse secondary antibody coupled with 6 nm gold (EMS). Finally, grids were xed with 1% glutaraldehyde (EMS), washed and embedded in a thin lm of uranyl acetate-methylcellulose (mixture of 4% uranyl acetate and 2% methylcellulose in 1:9 ratio) using the wire loop technique. Samples were analyzed under a JEOL transmission electron microscope (JEM-1010) operated at 80 kV and equipped with an SIA model 12C 4K CCD camera.
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