Malp2

All MAP kinase inhibitors, including SB203580, U0126, and SP600125, were obtained from Calbiochem (Darmstadt, Germany). Parthenolide, an NF-κB inhibitor, was purchased from Sigma-Aldrich Chemical Inc. (St. Louis, MO. USA). Antibodies specific for IκB-α, β-actin, and MAP kinases were from Cell Signaling Technology (Beverly, MA, USA). TLR1 and TLR6 neutralizing antibodies, P3C and CpG were purchased from InvivoGen (Cayla SAS, Toulouse, France). MALP2 was obtained from Alexis Biochemicals (San Diego, CA, USA). Enzyme-linked immunosorbent assay (ELISA) kits for TNF-α, IL-6, MCP-1, and IL-8 were purchased from Biolegend (San Diego, CA, USA) and MIP-1α ELISA kit was obtained from R&D systems (Minneapolis, MN, USA). Aluminum.

CpG (ODN M362) was purchased from Invivogen (United States), MALP-2 from Alexis Biochemicals (United States), and LPS from Salmonella minnesota from Enzo Life Sciences GmbH (Germany). All chemicals used were of analytical grade and received from commercial sources.