Propofol (YZ‐1572503) and D‐sorbitol (S8090) were obtained from Solarbio life science. Sorbitol level assay kits (MAK010) were purchased from Sigma. The SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) (9003) was from Cell Signaling Technology. Aldose reductase activity kit (Colorimetric) (ab273276), Antibodies targeting AKR1B1, AKR1B10, phosphorylated NRF2 at Ser40 and NRF2 were purchased from Abcam Company; antibodies targeting α‐tubulin and β‐actin and all unconjugated secondary antibodies were from Santa Cruz Biotechnology; PCNA (Abclonal, A0264), Histone 3 (Abclonal, A2348) and Ki‐67 (Abclonal, A16919) were obtained from Abclonal, and fetal bovine serum (FBS), Alexa‐488‐ and 594‐conjugated secondary antibodies were from Molecular Probes (Invitrogen). NRF2 plasmid was from Public Protein/Plasmid Library. The trizol was purchased from Invitrogen and All‐in‐One First‐Strand cDNA Synthesis Kit and All‐in‐One qPCR Mix were obtained from GeneCopoeia. Subcellular fractionation was conducted using NE‐PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher) following the manufacturer's recommendations. All ultrapure reagents were from Promega.
Unconjugated secondary antibodies
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Unconjugated secondary antibodies are antibodies that are not coupled with any reporter molecules or enzymes. They are designed to bind to the primary antibodies used in various immunoassays and detection techniques, allowing for the amplification and visualization of the target antigen.
Automatically generated - may contain errors
Lab products found in correlation
Cytoplasmic extraction reagent, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
All in one qpcr mix, by GeneCopoeia (1 mentions)
Proliferating cell nuclear antigen (pcna), by ABclonal (1 mentions)
Ne per nuclear, by Thermo Fisher Scientific (1 mentions)
D sorbitol, by Solarbio (1 mentions)
Histone 3, by ABclonal (1 mentions)
All in one first strand cdna synthesis kit, by GeneCopoeia (1 mentions)
P ikk, by Cell Signaling Technology (1 mentions)
Fibronectin, by Santa Cruz Biotechnology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
β catenin, by Santa Cruz Biotechnology (1 mentions)
Vimentin, by Santa Cruz Biotechnology (1 mentions)
N cadherin, by Santa Cruz Biotechnology (1 mentions)
Antibodies against zo 1, by Cell Signaling Technology (1 mentions)
E cadherin, by Santa Cruz Biotechnology (1 mentions)
P ikbα, by Cell Signaling Technology (1 mentions)
2 protocols using unconjugated secondary antibodies
1
Propofol and Sorbitol Regulation of NRF2
2
Comprehensive Antibody Profiling for Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies targeting E-cadherin, N-cadherin, β-catenin, fibronectin, vimentin, β-actin, and all unconjugated secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); Antibodies against ZO-1, p65, p-IKK, IKKβ, p-IKBα, IKBα, and IKKα were obtained from Cell Signaling Technology (Danvers, MA, USA); Antibody to RAP2C was purchased from Abcam (Cambridge, MA, USA); Antibody targeting β-tubulin was obtained from Ray Antibody (Beijing, China) and antibody to Histone H3 (K4) was purchased from Bioworld (St. Louis Park, MN, USA). All the primary antibodies were used at a dilution of 1:1000 in PBST Buffer (137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, 1.46 mM KH2PO4, 0.05% Tween-20) with 5% non-fat dry milk.
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