Anti rabif mouse monoclonal antibody d 12

For IF, cells were plated onto collagen IV‐coated glass coverslips (10 μg/ml) and then fixed with 4% paraformaldehyde (PFA) in PBS (phosphate‐buffered saline) for 15 min, permeabilized with 0.2% Triton X‐100 in PBS for 5 min, blocked with 5% goat serum in PBS for 1 h, and incubated with Rab13 rabbit polyclonal antibody (Novus Biologicals, cat# NBP1‐85799, 1/200 dilution) for 1 h. Secondary antibodies were conjugated with Alexa 647 (Thermo Fisher Scientific).
For Western blot detection, the following antibodies were used: anti‐Rab13 rabbit polyclonal (Novus Biologicals, cat# NBP1‐85799, 1/1,000 dilution), anti‐GFP rabbit polyclonal (Invitrogen, cat# A11122, 1/2,000 dilution), anti‐RhoGDI, anti‐transferrin receptor (clone H68.4, Thermo Fisher Scientific, cat# 13‐6800), anti‐α‐tubulin mouse monoclonal (Sigma‐Aldrich, cat# T6199, 1/10,000 dilution), anti‐GAPDH rabbit monoclonal 14C10 (Cell Signaling Technology, cat# 2118, 1/2,000 dilution), anti‐phospho‐FAK(Y397) (Cell Signaling Technology, cat# 3283), anti‐REP‐1 (Abcam, cat# 134964), anti‐RabGDI (Santa Cruz Biotechnology, cat# sc‐374649), and anti‐RABIF mouse monoclonal antibody D‐12 (Santa Cruz biotechnology, cat# sc‐390759, 1/1,000 dilution).