The apparatus comprised four identical cubes made of Plexiglas with a white opaque bottom, each measuring 40 × 40 × 40 cm. In the middle of the floor, a central arena (13.5 cm × 13.5 cm) was demarcated by a red line.31 Mice received 0.9% NaCl i.p., to control for injection stress, and were returned to the open field for 30 min. Afterwards, they were carefully removed, given an i.p. injection of amphetamine, and returned to the open field for a further 90 min. Amphetamine sulphate (Sigma, St Louis, MO, USA), was dissolved in 0.9% NaCl solution on the day of testing to obtain 2.5 mg kg−1, in a volume of 5 ml kg−1. Locomotor activity was recorded after SAL and amphetamine injection using Ethovision tracking system. The dependent measures were the total distance travelled during four time blocks: SAL (30 min), amphetamine (Amph)-block 1 (30 min), Amph-block2 (30 min) and Amph-block3 (30 min).
Amphetamine sulphate
Manufactured by Merck Group
Sourced in United States
Amphetamine sulphate is a chemical compound used in various laboratory and research applications. It is a white crystalline powder that serves as a stimulant. The core function of amphetamine sulphate is to act as a reagent or intermediate in chemical synthesis and analysis processes. No further details or interpretation on the intended use of this product can be provided.
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Lab products found in correlation
3 protocols using amphetamine sulphate
1
Amphetamine-Induced Locomotor Activity in Mice
2
Amphetamine-Induced Locomotor Activity
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Motor function was evaluated using an activity cage (43 × 43 × 32 cm) (Ugo Basile, Varese, Italy), placed in a sound-attenuating room. The cage was fitted with two parallel horizontal infrared beams located 2 cm from the floor. Before the start of the test, each mouse (6–7 weeks of age) was habituated to the testing room for at least 1 h. Cumulative horizontal movement counts were recorded for 4 h before and 3 h after treatment. Animals were treated subcutaneously with saline or amphetamine sulphate (4 mg kg−1) (Sigma-Aldrich) dissolved in 0.9% NaCl, according to Hess et al.29 (link)
3
Pharmacological Conditioning Agents Protocols
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Alcohol solutions were prepared from ethyl alcohol (200 Proof, OmniPur, EM Science; Gibbstown, NJ) in saline to a concentration of 0.2 g/ml, and injected via intraperitoneal (i.p.) route in a volume of 10 ml/kg body weight, yielding a dose of 2 g/ kg of ethanol. Nicotine free base from MP Biomedicals, Inc. (Solon, OH, USA) was dissolved in saline (0.025 mg/ml) and injected subcutaneously (s.c.) in a volume of 10 ml/kg body weight, yielding a dose of 0.25 mg/kg of Nicotine free base. Amphetamine sulphate (Sigma/Aldrich) solutions (0.1 and 0.3 mg/ml) were prepared in saline and injected (i.p.) in a volume of 10 ml/kg body weight to yield doses of 1 and 3 mg/kg, respectively. The solutions were made daily prior to each conditioning and on the test day for state-dependent conditioned place preference (CPP).
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