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Pcmv sp3 flag

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The PCMV-Sp3-FLAG is a plasmid vector that contains the human CMV promoter and the Sp3 transcription factor with a FLAG epitope tag. This vector is commonly used for the expression and detection of Sp3 in various cell lines.

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Lab products found in correlation

Pcmv sp3 flag, by GenScript (2 mentions) Quikchange site directed mutagenesis kit, by Agilent Technologies (2 mentions) Pegfp c2, by Takara Bio (2 mentions) Pflag cmv 2, by Merck Group (2 mentions)

2 protocols using pcmv sp3 flag

1

Construction and Characterization of Sp3 Expression Plasmids

Cited 2 times
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The expression plasmid pCMV-Sp3-FLAG was purchased from GenScript (no. OHu28260D; NM_003111.4; Homo sapiens Sp3, transcript variant 1, mRNA), and pCMV-GFP-Sp3 was constructed by inserting the full-length Sp3 cDNA into pEGFP-C2 (no. 6083-1; Clontech). Point mutations in pCMV-Sp3-FLAG or pCMV-GFP-Sp3 were created by using the QuikChange site-directed mutagenesis kit (no. 200518; Agilent Technologies). To construct the plasmids encoding F-Sp3 or deletion mutants, the corresponding Sp3 DNA fragments were amplified by PCR and then inserted into pFLAG-CMV-2 (no. E7398; Sigma-Aldrich). The plasmids including pCMV-FLAG-ORF50, pCMV-GFP-ORF50(1–590), pCMV-GFP-ORF50(1–490), pE4-luc, ORF56p(−97/−44), ORF21p(−194/−154), ORF60(−71/−32), and IL-10(−141/−102) have been described previously (23 (link), 64 (link), 65 (link)).
Chen L.Y., Chen L.W., Hung C.H., Lin C.L., Wang S.S, & Chang P.J. (2022). Caspase-Mediated Cleavage of the Transcription Factor Sp3: Possible Relevance to Cancer and the Lytic Cycle of Kaposi’s Sarcoma-Associated Herpesvirus. Microbiology Spectrum, 10(1), e01464-21.
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2

Construction and Characterization of Sp3 Expression Plasmids

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The expression plasmid pCMV-Sp3-FLAG was purchased from GenScript (no. OHu28260D; NM_003111.4; Homo sapiens Sp3, transcript variant 1, mRNA), and pCMV-GFP-Sp3 was constructed by inserting the full-length Sp3 cDNA into pEGFP-C2 (no. 6083-1; Clontech). Point mutations in pCMV-Sp3-FLAG or pCMV-GFP-Sp3 were created by using the QuikChange site-directed mutagenesis kit (no. 200518; Agilent Technologies). To construct the plasmids encoding F-Sp3 or deletion mutants, the corresponding Sp3 DNA fragments were amplified by PCR and then inserted into pFLAG-CMV-2 (no. E7398; Sigma-Aldrich). The plasmids including pCMV-FLAG-ORF50, pCMV-GFP-ORF50(1–590), pCMV-GFP-ORF50(1–490), pE4-luc, ORF56p(−97/−44), ORF21p(−194/−154), ORF60(−71/−32), and IL-10(−141/−102) have been described previously (23 (link), 64 (link), 65 (link)).
Chen L.Y., Chen L.W., Hung C.H., Lin C.L., Wang S.S, & Chang P.J. (2022). Caspase-Mediated Cleavage of the Transcription Factor Sp3: Possible Relevance to Cancer and the Lytic Cycle of Kaposi’s Sarcoma-Associated Herpesvirus. Microbiology Spectrum, 10(1), e01464-21.
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