Grp78 antibody

Manufactured by Novus Biologicals

GRP78 antibody is an immunoglobulin that specifically binds to the GRP78 protein, also known as BiP (Binding immunoglobulin Protein). GRP78 is a molecular chaperone that plays a key role in the endoplasmic reticulum (ER) stress response and protein folding process.

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Lab products found in correlation

Hrp conjugated anti rabbit and anti mouse secondary antibodies, by GE Healthcare (2 mentions) X box binding protein 1s xbp1s antibody, by BioLegend (2 mentions) Ccaat enhancer binding protein homologous protein chop antibody, by Thermo Fisher Scientific (2 mentions) Alexa 488 conjugated anti rabbit and alexa 594 conjugated anti mouse antibodies, by Thermo Fisher Scientific (2 mentions) Cleaved caspase 3, by Cell Signaling Technology (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (2 mentions) Cleaved caspase 12, by Cell Signaling Technology (1 mentions) P p70s6k, by Cell Signaling Technology (1 mentions) P perk, by Cell Signaling Technology (1 mentions) P eif2α, by Cell Signaling Technology (1 mentions) P 4ebp1, by Cell Signaling Technology (1 mentions) P70s6k, by Cell Signaling Technology (1 mentions) Rapamycin, by Merck Group (1 mentions) 4 hydroxynonenal hne adduct assay, by Cell Biolabs (1 mentions)

2 protocols using grp78 antibody

Quantification of Endoplasmic Reticulum Stress

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Reagents for blood ethanol levels were purchased from Analox Instruments (London, UK). ATF6 was purchased from Life Span Biosciences (Seattle, WA). Cleaved caspase-3 was from Cell Signaling Technology (Danvers, MA). GRP78 antibody was from Novus Biologicals (Littleton, CO). X-box binding protein-1s (XBP1s) antibody was from BioLegend (San Diego, CA). MANF antibody was from Abcam (Cambridge, MA). CCAAT/enhancer-binding protein homologous protein (CHOP) antibody was from Thermo Fisher Scientific (Rockford, IL). HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were from GE Healthcare Life Sciences (Piscataway, NJ). Mounting media containing 4′, 6-diamidino-2-phenylindole (DAPI) was from Vector Laboratories (Burlingame, CA). Alexa-488 conjugated anti-rabbit and Alexa-594 conjugated anti-mouse antibodies were from Life Technologies (Grand Island, NY). Ketamine/xylazine was from Butler Schein Animal Health (Dublin, OH). Other chemicals and reagents were purchased either from Sigma-Aldrich or Life Technologies (Frederick, MD).

Wang Y., Wen W., Li H., Clementino M., Xu H., Xu M., Ma M., Frank J, & Luo J. (2020). MANF is Neuroprotective against Ethanol-induced Neurodegeneration through Ameliorating ER Stress. Neurobiology of disease, 148, 105216.

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Measuring Alcohol and Cell Signaling

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We purchased the reagents for the measurement of ethanol from Analox instruments (London, UK). 4-Hydroxynonenal (HNE) adduct assay was from Cell Biolabs, Inc. (San Diego, CA). Rapamycin was from EMD Millipore (Burlington, MA). ATF6 was from LifeSpan Biosciences (Seattle, WA). mTOR, p-mTOR, 4EBP1, p- 4EBP1, p70S6K, p-p70S6K, eukaryotic initiation factor 2α (eIF2α), p-eIF2α, Ki-67, p- PERK, cleaved caspase-3, and cleaved caspase-12 antibodies, were from Cell Signaling Technology (Danvers, MA). GRP78 antibody was from Novus Biologicals (Littleton, CO). X-box binding protein-1s (XBP1s) antibody was from BioLegend (San Diego, CA). Doublecortin (DCX) antibody was from Abcam (Cambridge, MA). CCAAT/- enhancer-binding protein homologous protein (CHOP) antibody was from Thermo Fisher Scientific (Rockford, IL). HRP-conjugated anti-rabbit and anti-mouse secondary antibodies were from GE Healthcare Life Sciences (Piscataway, NJ). Mounting media containing 4’, 6-diamidino-2-phenylindole (DAPI) was from Vector Laboratories (Burlingame, CA). Alexa-488 conjugated anti-rabbit and Alexa-594 conjugated anti mouse antibodies were from Life Technologies (Grand Island, NY). Ketamine/xylazine was from Butler Schein Animal Health (Dublin, OH). Other chemicals and reagents were purchased either from Sigma-Aldrich or Life Technologies (Frederick, MD).

Wang Y., Wang X., Li H., Xu M., Frank J, & Luo J. (2018). Binge Ethanol Exposure Induces Endoplasmic Reticulum Stress in the Brain of Adult Mice. Toxicology and applied pharmacology, 356, 172-181.

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