Villin creert

Villin-Cre (Jackson Laboratories, Strain #: 021504, RRID:IMSR_JAX:021504), Villin-CreERT2^{27 (link)} (kind gift from Dr. Robine), and Lsd1f/f ^{69 (link)} (kind gift from Stuart Orkin) mice were housed in CoMed. Villin-Cre Lsd1f/f (cKO) mice were housed under specific-pathogen-free (SPF) conditions, and Villin-CreERT2 Lsd1f/f (icKO) mice were maintained in the minimal disease unit at CoMed. Mice were housed with controlled temperature between 21 and 22 degrees Celsius and relative humidity between 45 and 60%. The animals are housed in a 12-h dark/12-h light cycle, with 1 h of dusk/dawn. All mice used were between 8–14 weeks of age. By conventionally bred or conventionally raised animals, we refer to mice (of all genotypes) housed in these conditions and not treated with antibiotics.

Znhit1^fl/fl mice were generated by Model Animal Research Center of Nanjing University (MARC, Nanjing, China). The targeting strategy is shown in Supplementary Fig. 2a. Genotyping primer pair: sense 5′-GTTGGGAGCATCTGCCTTTC-3′, anti-sense 5′-CCCTGCCTACATCTGCACTAA-3′.
Villin-cre, Villin-creERT, and Lgr5-EGFP-IRES-creERT2 mice were obtained from the Jackson Laboratory. Olfm4-IRES-eGFPcreERT2 mice were provided by Hans Clevers. H2afv^fl/fl/H2afz^fl/fl mice were obtained from RIKEN BioResource Center. All strains were maintained in C57BL/6 background. For Cre induction, mice were intraperitoneally injected with 100 μl tamoxifen in sunflower oil at 20 mg/ml for 3–4 consecutive days.
All breeding and experimental procedures were performed in accordance with the relevant guidelines and regulations and with the approval of the Institutional Animal Care and Use Committee at Fudan University or Cincinnati Children’s Hospital.