Spss statistical program version 19

All analyzes were performed using the SPSS statistical program version 19.0 (IBM, São Paulo, Brazil). Initially, the normality of all salivary parameter data was evaluated by the Shapiro–Wilk test, followed by an analysis of the homogeneity of variance using the Levene test. Since all salivary parameters showed a deviation of normality, significant differences between the groups were evaluated using the non-parametric Kruskal–Wallis test with Dunn's post-test for multiple comparisons. Associations between clinical, sociodemographic and salivary parameters were tested through bivariate correlation and Spearman's coefficient. The significance cutoff level adopted in all analyzes was 5%.

For comparison of two groups, data were analyzed by Student's t-test, followed by Bonferroni's correction. One-way analysis of variance (ANOVA) was performed for three or more groups, followed by the LSD post hoc test. When more than one factor was examined simultaneously, data were analyzed by two-way ANOVA, followed by the LSD post hoc test. For body weight, data were analyzed by repeated measures two-way ANOVA. A Dunnett's T3 test was used to compare the differences between groups when equal variances were not assumed. P-values<0.05 were considered statistically significant and results were represented as means±s.e.m. Data analyses were performed using SPSS statistical program, version 19.0 (IBM, Armonk, NY, USA).