Fluorescent images were acquired at 20x and 40x using z-stacks on an LSM 710 laser scanning confocal microscope (Zeiss). Cell counting analysis was completed within FIJI. H&E stained lung sections were tile-scanned using a 4x objective on the Eclipse Ni series upright microscope (Nikon). Whole lobe immunofluorescence tile scans in Figure 5f were acquired using a 4x objective on the Nikon Eclipse Ni series upright microscope.
Jones D.L., Morley M.P., Li X., Ying Y., Cardenas-Diaz F.L., Li S., Zhou S., Schaefer S.E., Chembazhi U.V., Nottingham A., Lin S., Cantu E., Diamond J.M., Basil M.C., Vaughan A.E, & Morrisey E.E. (2024). An injury-induced tissue niche shaped by mesenchymal plasticity coordinates the regenerative and disease response in the lung. bioRxiv.
+ Open protocol