Anti hdac1 sc 7872

For analyses, we used the following primary antibodies: anti-METTL3 (A8370; AB Clonal); anti-METTL14 (A8530; AB Clonal); anti-HDAC1 (sc-7872; Santa Cruz Biotechnology, Dallas, TX, USA); anti-HDAC3 (SAB1404635, Prague, Sigma Aldrich); anti-H3K4me2 (07–030, Upstate, St. Louis, MO, USA); anti-H3K9me3 (ab8898, Abcam, Cambridge, UK); anti-α-actinin (A7811; Sigma-Aldrich, Prague, Czech Republic); and anti-troponin (ab47003, Abcam, Cambridge, UK). The following secondary antibodies were used for Western blot analyses: anti-rabbit IgG (A4914, Sigma Aldrich, Prague, Czech Republic) and anti-mouse IgG (A9044, Sigma Aldrich, Prague, Czech Republic). Protein concentration was measured by µQuant^TM microplate spectrophotometer (BioTek, see at https://www.artisantg.com/Scientific/71777-1/Bio-Tek-uQuant-Universal-Microplate-Spectrophoto-meter). Gels were stained by amido-black 10 B. The density of Western blot fragments was measured, and data were normalized to the level of total proteins. All blots were imaged on Amersham Imager 680. The final quantification of protein levels was performed using Image Studio Ver.5.2.

1,25D was purchased from Cayman Europe (Tallinn, Estonia), ATRA and curcumin were from Sigma. The compounds were dissolved in an absolute ethanol to 1000 × final concentrations, and subsequently diluted in the culture medium to the required concentration. IFNα (cat. no. 11343506), IFNγ (cat. no. 11343536) and antibodies CD11b-FITC (cat. no. 21279113) and CD14-PE (cat. no. 21270144), as well as appropriately labeled isotype controls were from ImmunoTools (Friesoythe, Germany). Mouse monoclonal anti-VDR (sc-13133), anti-Stat1 p84/p91 (sc-464) and anti-p-STAT1 (sc-8394), rabbit polyclonal anti-actin (sc-1616), anti-HDAC1 (sc-7872) and anti-Histone H1 (sc-10806) were from Santa Cruz Biotechnology Inc. (Santa Cruz Biotechnology Inc., CA). Goat anti-rabbit IgG, anti-mouse IgG conjugated to peroxidase, anti-mouse conjugated to biotin and streptavidin conjugated to peroxidase were from Jackson ImmunoResearch (West Grove, PA).