Tetracycline free serum

Manufactured by Takara Bio

Tetracycline-free serum is a laboratory reagent used as a supplement in cell culture media. It is a serum derived from bovine or other animal sources that has been processed to remove tetracycline and other antibiotics. This product is intended to provide a defined, antibiotic-free component for the cultivation of cells in vitro.

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Lab products found in correlation

Doxycycline, by Takara Bio (1 mentions) Bi2536, by Chemietek (1 mentions) Pen strep, by Thermo Fisher Scientific (1 mentions) Glutamax, by Thermo Fisher Scientific (1 mentions) Dmem medium, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

2 protocols using tetracycline free serum

Adenoviral Overexpression and Lentiviral Knockdown

Cited 3 times

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Murine Xbp-1s and murine Atf4 adenoviruses used here were previously described[32 (link), 33 (link)]. The shRNA lentivirus plasmids were packaged by transfection into 293T cells, as already described[34 (link)]. Stably transduced cells were selected in media containing puromycin (5μg/ml). Conditional shRNA were selected and maintained in media containing tetracycline-free serum (Clontech).

D’Osualdo A., Anania V.G., Yu K., Lill J.R., Kaufman R.J., Matsuzawa S.I, & Reed J.C. (2015). Transcription Factor ATF4 Induces NLRP1 Inflammasome Expression during Endoplasmic Reticulum Stress. PLoS ONE, 10(6), e0130635.

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Characterization of Glioblastoma Cell Lines

Cited 1 time

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The U87MG parental, U87MG EGFRvIII, U178MG tet-EGFRvIII cell lines, and transformed mouse Ink4a/Arf(−/−) astrocytes, Ink4a/Arf(−/−) EGFRvIII cells, Ink4a/Arf(−/−) EGFRvIII Gefitinib/Erlotinib resistant cells, and Ink4a/Arf(−/−) PDGF-β cells have been previously described [29 (link), 31 (link), 42 (link)]. U87MG parental H2B-GFP and U87MG EGFRvIII H2B-GFP cells were constructed by infecting U87MG parental and U87MG EGFRvIII cells with a H2B-GFP construct [43 (link)] generously provided by Dr. David Pellman (Dana Farber Cancer Institute, Boston). TMZ (AK Scientific, Mountain View, CA) and BI2536 (ChemieTek, Indianapolis, IN) were dissolved in DMSO (Sigma Aldrich, St. Louis, MO). Doxycycline (Clontech, Mountain View, CA) was dissolved in deionized water. Cells were cultured in DMEM medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco), 1% Pen-Strep (Gibco) and 1% GlutaMax (Gibco) unless otherwise specified. Tetracycline free serum (Clontech, Mountain View, CA) was used for experiments involving Doxycycline.

Shen Y., Li J., Nitta M., Futalan D., Steed T., Treiber J.M., Taich Z., Stevens D., Wykosky J., Chen H.Z., Carter B.S., Becher O.J., Kennedy R., Esashi F., Sarkaria J.N., Furnari F.B., Cavenee W.K., Desai A, & Chen C.C. (2015). Orthogonal targeting of EGFRvIII expressing glioblastomas through simultaneous EGFR and PLK1 inhibition. Oncotarget, 6(14), 11751-11767.

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