Rhfgf b

Manufactured by Lonza

Sourced in United States

RhFGF-B is a recombinant human fibroblast growth factor-beta (rhFGF-B) product. It is a purified protein that can be used for various research applications.

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Lab products found in correlation

5 protocols using rhfgf b

Isolation and Culture of HUVECs

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Human Umbilical Vein Endothelial Cells (HUVECs) were isolated using collagenase type-II (Sigma Aldrich, USA) digestion from human umbilical cord. The cells were cultured in 0.1% gelatin coated T-25 flask using endothelial growth medium-2 (EGM-2) supplemented with 2% FBS, VEGF, rhEGF, rhFGF-B, R3-IGF-1, gentamicin sulphate, amphotericin, hydrocortisone, heparin and ascorbic acid (Lonza, Switzerland). Cells between 2^nd to 6^th passages were used for the experiments as indicated^{36 (link)}.

Nareshkumar R.N., Sulochana K.N, & Coral K. (2018). Inhibition of angiogenesis in endothelial cells by Human Lysyl oxidase propeptide. Scientific Reports, 8, 10426.

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HUVEC Culture and Maintenance Protocol

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HUVECs were purchased from Vec Technologies (Rensselaer, NY). They were cultured in growth medium (EGM-2 plus rhEGF 0.5 mL, ascorbic acid 0.5 mL, hydrocortisone 0.2 mL, heparin 0.5 mL; VEGF 0.5 mL; GA-1000 0.5 mL, R3-IGF-1 0.5 mL, rhFGF-B 2.0 mL) from Lonza (Walkersville, MD) supplemented with 10% of fetal bovine serum (FBS) from Gemini Bio-Products (West Sacramento, CA) and used at passages 4–6.

Potje S.R., Chen Z., Oliveira S.D., Bendhack L.M., da Silva R.S., Bonini M.G., Antoniali C, & Minshall R.D. (2017). Nitric oxide donor [Ru(terpy)(bdq)NO]3+ induces uncoupling and phosphorylation of endothelial nitric oxide synthase promoting oxidant production. Free radical biology & medicine, 112, 587-596.

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Culturing Normal Human Lung Fibroblasts

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Normal human lung fibroblasts (NHLFs, CRL1490 cells from ATCC, Manassas, VA, USA) were cultured in fibroblast basal medium supplemented with 2% fetal bovine serum (FBS), 0.1% recombinant human fibroblast growth factor basic (rhFGF-B), 0.1% insulin, and 0.1% gentamicin/amphotecin-B (Lonza, Walkersville, MD, USA), and used in passages 3–6.
NHFLs were starved in the serum-free medium for 18 h to induce cell cycle synchronization,^{49 (link)} and then refreshed with complete culture medium. The cells were seeded on the substrates at a density of 4000 cells/cm² and cultured at 37 °C and 5% CO₂ for a predefined period of time before the analyses.

Wang K., Bruce A., Mezan R., Kadiyala A., Wang L., Dawson J., Rojanasakul Y, & Yang Y. (2016). Nanotopographical Modulation of Cell Function through Nuclear Deformation. ACS applied materials & interfaces, 8(8), 5082-5092.

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Culturing Normal Human Lung Fibroblasts

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NHLFs (CRL1490 cells; ATCC, Manassas, VA, USA) were cultured in fibroblast basal medium supplemented with 2 % fetal bovine serum (FBS), 0.1% recombinant human fibroblast growth factor basic (rhFGF-B), 0.1% insulin, and 0.1% gentamicin/amphotecin-B (Lonza, Walkersville, MD, USA), and used in passages 3 – 6.

Wang K., He X., Linthicum W., Mezan R., Wang L., Rojanasakul Y., Wen Q, & Yang Y. (2017). Carbon Nanotubes Induced Fibrogenesis on Nanostructured Substrates. Environmental science. Nano, 4(3), 689-699.

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Cell Culture Protocols for Diverse Cell Lines

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MEFs, PANC-1, A549, A375, A549/NF-κB-Luc and HeLa/NF-κB-Luc cells were cultured in Gibco high glucose DMEM (Life Technologies) with 10% FBS. G-361 cells were cultured in McCoy’s 5A medium (Life Technologies) with 10% FBS. All other cell lines were cultured in RPMI 1640 medium with 10% FBS. Culture media for all cell lines was supplemented with 1% penicillin/streptomycin. Media for A549/NF-κB-Luc and HeLa/NF-κB-Luc cells also contained 0.1 mg/mL hygromycin-B. NHDF and NHLF cells were cultured in fibroblast basal medium (FBM) supplemented with 0.1% insulin, 0.1% rhFGF-B, 0.1% GA-1000, and 2% fetal bovine serum (Lonza). NHMCs were cultured in mesangial cell basal growth medium (MsBM) supplemented with 5% fetal bovine serum and 0.1% GA-1000 (Lonza). Cells were cultured in a humidified atmosphere at 37°C with 5% CO₂. RTA 408 and bardoxolone methyl were dissolved in DMSO (vehicle). The final amount of DMSO in the media was ≤ 0.1% and was equivalent in drug- and vehicle-treated samples.

Probst B.L., Trevino I., McCauley L., Bumeister R., Dulubova I., Wigley W.C, & Ferguson D.A. (2015). RTA 408, A Novel Synthetic Triterpenoid with Broad Anticancer and Anti-Inflammatory Activity. PLoS ONE, 10(4), e0122942.

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