Cd3 amcyam clone sk7

Manufactured by BD

Sourced in United States

The CD3 AmCyan (clone SK7) is a fluorochrome-conjugated monoclonal antibody that binds to the CD3 antigen expressed on the surface of T cells. It is designed for use in flow cytometry applications to identify and quantify T cell populations.

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Lab products found in correlation

Diva software, by BD (2 mentions) Rainbow calibration particles, by BioLegend (1 mentions) Cd16 pacblue, by BD (1 mentions) Cd8 apc cy7, by BD (1 mentions) 7 color setup beads, by BD (1 mentions) Hla dr percp l243, by BD (1 mentions) Facs lysing solution, by BD (1 mentions) Cd86 pe it2, by BD (1 mentions) Cd38 apc hb 7, by BD (1 mentions) Facscanto flow cytometer, by BD (1 mentions) Cd4 pe cy7, by BD (1 mentions) Facsflow, by BD (1 mentions)

2 protocols using cd3 amcyam clone sk7

Quantifying KIR Expression on T and NK Cells

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The expression of KIR receptors (KIR2DL1, 2DS1, 2DL2/S2, 2DL3 and 3DL1) on CD3+CD8+ T cells and CD3–CD56+ NK cells in peripheral blood was evaluated as a percentage of positive cells using LSR-II and DIVA Software (BD, San Diego, CA, USA), as previously described [18 (link),20 (link)]. LSR-II photomultiplier (PMT) voltages were adjusted daily using rainbow calibration particles (BioLegend, San Diego, CA, USA). Fluorescence compensation was finely adjusted using negative events for each fluorochrome as a reference. The staining protocol consisted of a 11-color/12-monoclonal antibody (mAb) panel: CD3 AmCyam (clone SK7, BD), CD4 PE-CF594 (RPA-T4, BD), CD8 APCCy7 (SK1, BD), CD16 PacBlue (3G8, BD), CD56 BV711 (NCAM16.2, BD), CD158a,h PECy7 (EB6, BD, recognizes both KIR2DL1 and 2DS1), CD158b1/b2,j PE-Cy5 (GL183, Beckman-Coulter, Brea, CA USA, recognizes KIR2DL2, 2DL3 and 2DS2), CD158a FITC (143211, R&D Systems, MN, USA; KIR2DL1), CD158b2 APC (180701, R&D Systems, KIR2DL3), CD158e APC (DX9, R&D Systems, KIR3DL1), CD226 PE (11A8, BioLegend), and NKG2A biotin (REA110, Miltenyi Biotech, BergischGladbach, Germany).
The gating strategy used to identify total lymphocytes, CD3+, CD4+, and CD8+ T lymphocytes, as well as CD16^−/+CD56⁺⁺ (CD56^bright) and CD16⁺CD56⁺ (CD56^dim) NK cells is shown in Figure S2.

Gimeno L., Serrano-López E.M., Campillo J.A., Cánovas-Zapata M.A., Acuña O.S., García-Cózar F., Martínez-Sánchez M.V., Martínez-Hernández M.D., Soto-Ramírez M.F., López-Cubillana P., Martínez-Escribano J., Martínez-García J., Corbalan-García S., Álvarez-López M.R, & Minguela A. (2020). KIR+ CD8+ T Lymphocytes in Cancer Immunosurveillance and Patient Survival: Gene Expression Profiling. Cancers, 12(10), 2991.

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Multicolor Flow Cytometry of Immune Cell Activation

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EDTA anticoagulated peripheral blood cells were labeled following a lyse/wash protocol with an 8-color/9-monoclonal antibody (mAb) panel including CD3 AmCyam (clone SK7, BD Biosciences), CD4 PECy7 (SK3, BD), CD8 APCCy7 (SK1, BD), CD16 PacBlue (3G8, BD), CD19 PECy7 (SJ25C1, BD), CD28 FICT (CD28.2, BD), CD38 APC (HB7, BD), CD86 PE (IT2.2, BD), and HLA-DR PerCP (L243, BD). Five microliters of each antibody in 100 µL of whole blood was incubated for 15 minutes at room temperature in the dark. Samples were lysed with 3 mL of 1X FACSlysing solution (BD) for 5 minutes and washed with 3 mL of FACSFlow (BD). Half a million cells were immediately acquired in a FACSCanto flow cytometer (BD), daily calibrated using 7-color setup beads (BD), and analyzed with DIVA software (BD) following the gating strategy described in Supplementary Figure 1.
The expression of CD28, CD38, CD86, and HLA-DR activation/senescence markers was evaluated as a percentage or absolute number (cells/µL) of positive cells as well as mean fluorescence intensity (MFI) of the marker on CD3⁺CD4⁺, CD3⁺CD8⁺, and CD3⁺CD4⁺CD8⁺ T lymphocytes, CD19⁺ B lymphocytes, CD3^-CD19^-CD16⁺ natural killer (NK) lymphocytes, monocytes (CD4⁺CD86⁺HLA-DR⁺ medium side scatter [SSC] cells), granulocytes (CD16⁺⁺ elevated SSC cells), and eosinophils (elevated SSC auto fluorescent cells).

Bernal E., Martinez M., Campillo J.A., Puche G., Baguena C., Tomás C., Jimeno A., Alcaraz M.J., Alcaraz A., Muñoz A., Oliver E., de la Torre A., Marín I., Cano A, & Minguela A. (2021). Moderate to Intense Physical Activity Is Associated With Improved Clinical, CD4/CD8 Ratio, and Immune Activation Status in HIV-Infected Patients on ART. Open Forum Infectious Diseases, 9(3), ofab654.

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