Benzene concentrations were determined using a HPLC method (Bahrami et al., 2011 ) on a Waters device equipped with a 1525 binary mixing pump, a 717 plus autosampler, a XBridge C18 (4.6 mm × 50 mm, 3.5 μm) column, and 2487 UV/Visible detector (Waters® Corporation, Milford, MA). The Breeze® software was used to control the system and collect data. Chemical separation was achieved with a mobile phase of 66% acetonitrile and 34% water at 1.0 mL/min flow rate with an injection volume of 25 μL. The retention time for benzene was 1.2 min. A linear standard curve of benzene was derived using a blank solution and a range of 9 benzene concentrations from 0.5 to 30 ppm (r2 = 0.999). The limit of detection was 500 ppb.
1525 binary mixing pump
Manufactured by Waters Corporation
Sourced in United States
The 1525 binary mixing pump is a laboratory equipment designed for precise fluid delivery and mixing. It features two independent pumping channels that can be used to accurately blend and proportion two different solutions or solvents. The pump offers programmable flow rates and volumes to support a variety of analytical applications. Its core function is to provide controlled and consistent liquid delivery for sample preparation, chromatography, and other laboratory processes.
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2 protocols using 1525 binary mixing pump
1
HPLC Quantification of Benzene
2
Determination of BTX by HPLC-UV
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BTX concentrations were determined at room temperature on a Waters high-performance liquid chromatograph (HPLC) equipped with a 1525 binary mixing pump, a 717 plus autosampler, an XBridge C18 column (4.6 mm × 50 mm, 3.5 μm), and a 2487 UV/Visible detector (Waters®® Corporation, Milford, MA, USA) [51 ]. An isocratic mobile phase of 66% acetonitrile and 34% water at 1 mL/min flow rate was programmed with an injection volume of 25 μL. Chemicals were qualified and quantified by the UV detector set at 254 nm and 202 nm. The retention time for BTX was between 1.2 min and 1.5 min. The Breeze®® software was used to control the system and collect data. A linear standard curve of each chemical was derived using a blank mobile phase and 9 concentrations from 0.5 to 30 μg/mL in water (r2 = 0.999). The limit of detection and limit of quantification for BTX were 3.5 and 10.7 μg/mL, respectively.
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