Anti histone h3

Primary antibodies for human DVL2 (1:1,000; cat. no. 3224), β-catenin (1:1,000; cat. no. 8480), cyclin D1 (1:1,000; cat. no. 2978), vimentin (1:1,000; cat. no. 5741), E-cadherin (1:1,000; cat. no. 3195), N-cadherin (1:1,000; cat. no. 13116), Snail (1:1,000; cat. no. 3879), and matrix metalloproteinase (MMP-9) (1:1,000; cat. no. 13667) were purchased from Cell Signaling Technology, Inc. Horseradish peroxidase (HRP)-conjugated anti-mouse IgG (1:1,000; cat. no. 7076) and anti-rabbit IgG (1:1,000; cat. no. 7074) secondary antibodies were purchased from Cell Signaling Technology, Inc. The control antibody (anti-GAPDH and anti-Histone H3) was acquired from Bioworld Technology, Inc. and Proteintech Group, Inc., respectively. Anti-human DVL2 (1:100; cat. no. sc-390303) was acquired for immunohistochemistry from Santa Cruz Biotechnology, Inc. Cell counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. TRIzol^® reagent and PrimeScript RT Master Mix (Perfect Real Time) were obtained from Takara Bio Inc.

Protein isolation from whole cerebral cortex or cells was performed as descried previously³³ (link). The concentration of protein was assessed by BCA assay kit (Thermo Fisher). Western blots were performed using the standard SDS-polyacrylamide gel electrophoresis method and electrotransferred onto PVDF membranes (Millipore, Billerica, MA, USA). After blocking with 3% (w/v) BSA (SunShine, Nanjing, China) in PBS for 2 h, the membranes were incubated with primary antibodies at 4 °C overnight, and then incubated with the corresponding secondary antibodies (CST, Boston, MA, USA; 1:10,000) for 1 h at room temperature. At last, the immunoblot was visualized and quantified by a gel densitometric scanning and analysis system (Bio-Rad, Berkeley, CA, USA).
Primary antibodies were used as follows: anti-SIRT6 (#12486; CST; 1:1000), anti-H3K9Ac (#9469; CST; 1:1000), anti-H3K56Ac (#4232; CST; 1:1000), anti-EZH2 (#5246; CST; 1:1000), anti-FOXC1 (ab227977; abcam, Cambridge, UK; 1:1000), anti-Pan Ac-K (A2391; abclonal, Wuhan, Hubei, China; 1:1000), anti-H3K14Ac (A7254; abclonal; 1:1000), anti-H3K27me3 (A2363; abclonal; 1:1000), anti-Histone H3 (BS90642; Bioworld Technology, Louis Park, MN, USA; 1:1000), anti-β-Actin (23660-1-AP; Proteintech, Wuhan, Hubei, China; 1:10,000).